抗CD105单克隆抗体对大肠癌生长的抑制作用  

Inhibitory effects of anti-CD105 monoclonal antibody on large intestine carcinoma cell xenografts in nude mice

作　　者：梁启廉[1] 李志东[2] 吴斌华[2] 王晖[2] 潘达超[1] 李姝君[1] 

机构地区：[1]广东医学院附属医院肿瘤中心,广东湛江524001 [2]广东医学院实验动物中心

出　　处：《中华实验外科杂志》2009年第2期165-166,共2页Chinese Journal of Experimental Surgery

基　　金：基金项目：广东省医学科研基金资助项目（A2003563）

摘　　要：目的观察抗CD105单克隆抗体对大肠癌生长的影响。方法建立裸小鼠人类大肠癌细胞动物模型，将44只裸小鼠随机分为2组，分别为抗体组和生理盐水组，抗体组在接种大肠癌细胞的同时，经腹腔注射抗CD105单克隆抗体，生理盐水组在接种大肠癌细胞的同时，经腹腔注射生理盐水，计算肿瘤体积和抑瘤率，并做组织病理学检查。结果抗CD105单克隆抗体可抑制人类大肠癌细胞株LoVo的生长，最高抑瘤率为71．88％，抗体组与生理盐水组比较肿瘤明显受抑制（P〈0．05）。结论抗CD105单克隆抗体对人类大肠癌细胞株LoVo的生长有明显抑制作用。Objective To observe the inhibitory effect of anti-CD105 monoclonal antibody on tumor growth of large intestine carcinoma. Methods We established an animal model of human large intestine carcinoma in nude mice, and inhibitory effects of anti-CD105 monoclonal antibody were tested. Forty-four nude mice with human large intestine carcinoma were randomly divided into 2 groups：In antibody group the mice were treated with monoclonal antibody, and in normal saline group the mice were given normal saline. The tumor volume and the tumor growth inhibition rate were calculated, and the tumor was his- topathlogically examined. Results The growth of human large intestine carcinoma cell line LoVo cells in antibody group was dramatically inhibited. As compared with the normal saline group, growth of the tumor was significantly reduced in size in the mice treated with anti-CD105 monoclonal antibody, with an inhibition rate of 71.88% （P 〈 0.05）. Conclusion The anti-CD105 monoclonal antibody had a significant inhibitory effect on the growth of large intestine carcinoma in mice.

关 键 词：大肠癌 单克隆抗体 CD105 

分 类 号：R735.34[医药卫生—肿瘤] R392-33[医药卫生—临床医学]