翻白草多糖的提取和鉴定  

Study on extraction and Identification of polysaccharides from Potentilla discolor.bunge.

作　　者：李胜华[1] 伍贤进[1] 

机构地区：[1]湖南省怀化学院生物工程系,怀化418008

出　　处：《食品科技》2009年第1期177-179,共3页Food Science and Technology

基　　金：科技部国家科技基础条件平台项目(2004DKA30430)

摘　　要：目的:筛选翻白草多糖的提取工艺和多糖的组成。方法:超声波法提取、正交实验优化、分光光度法测定含量及气相色谱法鉴定翻白草多糖的组成。结果:翻白草水溶性多糖的提取最佳工艺是固液比为1∶20、超声时间为30min、超声温度为60℃、醇析乙醇含量为95%;气相色谱鉴定多糖组分为鼠李糖、阿拉伯糖、甘露醇、半乳糖、木糖。Objective： Chance the best extraction process of polysaccharides and polysaccharide components from Potentilla discolor Bge. Methods： Supersonic extraction was used to extract. The optimum extracting process was selected by the orthogonal test. The polysaccharide component by GC. Results： The optimum condition of supersonic extraction was as follow： proportion of extracting solvents was 1：20, extracting time was 30 min, extracting temperature was 60 ℃ and extracting ethanol concentration was 95%, The components of the polysaccharide were identified by GC, The polysaccharide was assessed to be a heteroglycan composed of rhamnose, arabinose, mannitol, galactose, xylose.

关 键 词：翻白草 多糖 提取 气相色谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]