二价金属离子影响乳酸脱氢酶活性的单扫伏安研究  

Studies on the Effects of Five Kinds of Divalent Metal Ions on LDH Activity by Single Sweep Voltammetry

作　　者：汪勋清[1] 高小霞[1] 

机构地区：[1]北京大学化学系

出　　处：《高等学校化学学报》1998年第4期543-546,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：探讨了５种二价金属离子（Ｚｎ２＋，Ｃｕ２＋，Ｍｎ２＋，Ｃａ２＋，Ｍｇ２＋）对乳酸脱氢酶（ＬＤＨ）在辅酶ＮＡＤＨ存在下转化丙酮酸为乳酸催化体系的影响，结果表明，Ｚｎ２＋、Ｃａ２＋对ＬＤＨ活性无影响，Ｍｎ２＋有抑制作用，而Ｃｕ２＋、Ｍｇ２＋在低浓度时有微弱的促进作用．从Ｚｎ２＋与辅酶、酶的三元配合物的模型可以看出，ＬＤＨ是一非金属酶，Ｚｎ２＋不起催化作用．对Ｃｕ２＋、Ｍｇ２＋、Ｃａ２＋。In this paper the effects of five kinds of divalent metal ions on LDH activity were studied. The results showed that Zn 2+ and Ca 2+ do not affect LDH activity while Mn 2+ exerted inhibitory effect. At low concentrations, Cu 2+ and Mg 2+ slightly stimulate LDH activity. From the binding model of ternary complex, it could be concluded that LDH is a non metallic enzyme and Zn 2+ did not play a catalytic role in LDH. The different effects of Cu 2+ , Mg 2+ , Mn 2+ and Ca 2+ ions were also discussed.

关 键 词：LDH NADH 活性 乳酸 丙酮酸 二价金属离子 催化 

分 类 号：Q554.02[生物学—生物化学] O629.8[理学—有机化学]