红海榄根部盐胁迫反应的比较蛋白质组学分析  

Comparative Proteomic Analysis of Salt-stress Response Proteins in Rhizophora Stylosa Roots

作　　者：范吉星[1,2] 邓用川[1,3] 黄惜[1,3,2] 林栖凤[1,3] 罗越华[1,2] 

机构地区：[1]海南大学农学院,海口570228 [2]海南大学教育部热带生物资源重点实验室,海口570228 [3]海南省耐盐作物生物技术重点实验室,海口570228

出　　处：《中国生物化学与分子生物学报》2009年第1期72-77,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：海南省重点科技项目(No.04103);海南省自然科学基金项目(No.808116);海南省重点学科建设项目;海南省耐盐作物生物技术重点实验室开放基金资助~~

摘　　要：红海榄（Rhizophora stylosa）是一种典型的红树林盐生植物.本研究利用蛋白组学技术对淡栽（R0）和3%NaCl盐栽（R3）处理后的红海榄根部总蛋白进行了比较研究.双向电泳图谱的结果表明,R0和R3分别有981和972个蛋白点,蛋白点主要集中在分子量28-70 kD,等电点4.0-8.5之间.R0和R3之间差异明显的有15个蛋白点.其中,8个蛋白的表达量在R0中表达增高（10倍）,而在R3中相对下降.另外,7个蛋白的表达量在R0中较低,而在R3中表达量显著增高.对这15个蛋白点进行肽质量指纹图谱分析,10个蛋白点找到匹配蛋白.功能预测分析发现,在盐水栽培上调的蛋白质一般与逆境胁迫有关,淡水栽培上调的蛋白质一般与基本代谢有关.这些研究结果为进一步研究红海榄的耐盐机理提供了有意义的线索.Rhizophora stylosa is a typical halophyte in mangrove forests. In this study, we analyzed the differential expression of salt-stress responsive proteins in the roots of Rhizophora stylosa treated with fresh water （R0） or 3 % NaCl solution （ R3 ） . Two-dimensional electrophoresis （2-DE） showed that 981 and 972 protein spots were identified in respect to different conditions, and most proteins with pI 4.0--8.5 and molecular weight ranged at 28--70 kD. 15 proteins were found to be differentially expressed in Ro and R3, 8 of which were up-regulated （10 fold） in Ro, and 7 in R3 .MALDI-TOF-MS analysis showed that 10 out of the 15 proteins could be identified through peptide mass fingerprint （PMF）. The proteins up-regulated in R3 were associated with the stress-induction, whereas those highly expressed in Ro were related to the basic metabolism. These results may provide important information to understand the molecular mechanisms of salt-tolerant in Rhizophora stylosa.

关 键 词：红海榄 盐胁迫反应 比较蛋白组学 双向电泳 质谱 

分 类 号：Q71[生物学—分子生物学]