荧光原位杂交和免疫组织化学方法在检测间变性大细胞淋巴瘤ALK基因转位及其融合蛋白的对比研究  

Detection of ALK gene translocation by fluorescence insitu hybridization and immunohistochemistry anaplastic large cell lymphoma tissue and its significance

作　　者：季天海[1] 李慧灵[2] 蒋会勇[2] 赵彤[2] 余英豪[1] 

机构地区：[1]福州总医院病理科 [2]南方医科大学附属南方医院病理科

出　　处：《福州总医院学报》2008年第B12期342-344,共3页Journal of Fuzhou General Hospital

基　　金：广东省社会发展攻关基金资助项目(B30301);广州市科技计划基金资助项目(2002Z34061)

摘　　要：目的:比较荧光原位杂交(fluorescence insitu hybridization,FISH)和免疫组织化学在检测间变性大细胞淋巴瘤(anaplasticlargecelllymphoma,ALCL)中间变性淋巴瘤激酶(anaplasticlymphomakinase,ALK)基因转位及其融合蛋白中的作用。方法:采用双色FISH和免疫组织化学检测30例石蜡包埋ALCL病例中ALK基因转位及其融合蛋白。结果:FISH和免疫组织化学分别在60.7%(17/28)、71.4%(20/28)系统性ALCL中检测到ALK基因转位或融合蛋白,在2例皮肤原发ALCL中均未检测到基因转位或融合蛋白。结论:在检测ALCL中有无ALK基因转位时,ALK蛋白免疫组化由于其简单、快捷、价廉成为一般情况下的首选方法;在具备FISH条件时,由于其准确性也可以将检测ALK基因转位作为首选。Objective： To compare the value of fluorescence insitu hybridization （FISH） with that of immunohistoehemistry in detecting ALK gene translocations and ALK fusion protein in anaplastic large cell lymphoma （ALCL） . Method： Dual - color FISH and ALK-1 immunohistoehemistry were used to detect ALK gene translocation and ALK fusionprotein in 30 ALCL eases. Results： ALK gene translocations and ALK fusion protein expression were detected in 17 or 20 of the 28 systemic ALCL; it was not detected in 2 primary cutaneous ALCL.Conclusion： Immunohistoehemieal method is the first choice in detecting ALK gane translocation, but when conditions permit, FISH can be the first choice for its aeeur.

关 键 词：间变性大细胞淋巴瘤 间变性淋巴瘤激酶 荧光原位杂交 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤] R733.4[医药卫生—临床医学]