膜联蛋白-1在大肠癌组织中的表达及意义  

Expression and significance of AnnexinⅠin colorectal carcinomas

作　　者：曲利娟[1] 梁莉[2] 丁彦青[2] 

机构地区：[1]南京军区福州总医院病理科 [2]南方医科大学病理学教研室

出　　处：《福州总医院学报》2008年第B12期361-364,共4页Journal of Fuzhou General Hospital

摘　　要：目的:探讨大肠癌组织中膜联蛋白-1(AnnetinⅠ)的表达及意义。方法:应用原位杂交和免疫组化方法,从mRNA及蛋白两个水平上检测95例大肠癌及35例正常大肠黏膜组织AnnexinⅠ的表达情况。结果:大肠癌组An- nexinⅠmRNA表达较正常组下调,而大肠癌组AnnexinⅠ蛋白表达较正常组上调,两组表达差异分别具有显著性(P<0.01)。在mRNA水平,表达与肿瘤分化程度正相关(P<0.05),与有无淋巴结转移和组织学类型无关。在蛋白水平,表达与淋巴结转移正相关(P<0.05),与组织学类型和分化程度无关。原发癌与淋巴结转移癌两个水平表达差异均无显著性(P>0.05)。结论:AnnexinⅠ与大肠癌发生发展和转移密切相关,可作为反映大肠癌生物学行为和判断预后的重要指征。Objective： To investigate the expression and signaficance of AnnexinⅠ in colorectal carcinomas. Method： 95 eases of eolorectal carcinoma tissues and 35 cases of normal colorectal tissues were examined by immunohistoehemieal staining and in situ hybridization Results： The expression of AnnexinⅠ mRNA was significantly lower in eolorectal carcinoma group than that in normal group （P〈 0.01 ） . The expression of AnnexinⅠ protein was significantly higher in colorectal carcinoma group than that in normal group （P〈0.01） . The expression of AnnexinⅠ mRNA was positively related to differentiation grades （P〈（0.05）, but there was no closely related to lymph node metastasis and histologlcal types. The expression of AnnexinⅠ protein was positively related to lymph node metastasis （P〈0.05）, but there was no closely related to histological types and differentiation grades. There existed no significant difference between in situ tumor and in metastatic tumor of colorectal carcinomas both mRNA and protein level （ P 〉 0.05） . Conclusion; AnnexinⅠ is closely related to tumorigenesis, progression and metastasis in colorectal carcinomas, which is an important indication for the biological behaviors and prognosis of colorectal carcinomas.

关 键 词：大肠癌 AnnexinImRNA 蛋白质ArmexinⅠ 转移 

分 类 号：R730.23[医药卫生—肿瘤] R735.34[医药卫生—临床医学]