实验性糖尿病牙周炎骨丧失动物模型研究  

Study of experimental diabetic periodontitis-associated bone loss in rats

作　　者：付永伟[1,2] 和红兵[1] 欧炯光[1] 

机构地区：[1]云南昆明医学院,650031 [2]江苏省连云港市第一人民医院口腔科,222002

出　　处：《实用口腔医学杂志》2009年第1期13-17,共5页Journal of Practical Stomatology

摘　　要：目的:建立实验性糖尿病牙周炎骨丧失动物模型,以进一步揭示糖尿病加重牙周炎骨丧失的细胞学机制。方法:选用6周龄雄性SD大鼠62只,随机分为糖尿病牙周炎组(DP)、牙周炎组(P)以及正常对照组(N)。采用一次性腹腔注射链脲佐菌素(streptozotocin,STZ)的方法诱导大鼠糖尿病模型,采用丝线结扎联合口内接种细菌的方法建立牙周炎模型。动物分别于丝线结扎后3周和6周分批处死,进行HE染色、TRAP染色。观测指标包括:牙槽骨丧失,组织病理学比较,炎症区破骨细胞计数等。资料采用单因素方差分析统计学处理。结果:丝线结扎后3周和6周,大鼠牙槽骨丧失在N组与P组、N组与DP组、P组与DP组不同,组间两两比较均有统计学差异(P<0.05),牙槽骨丧失DP组>P组>N组。炎症区单位长度破骨细胞数在N组、P组、DP组不同,N组与P组比较,N组与DP组比较、P组与DP组比较均有统计学差异(P<0.05),其炎症区单位长度破骨细胞数DP组>P组>N组。结论:糖尿病可加重牙周炎牙周组织破坏,糖尿病条件下牙周炎骨丧失明显增加。糖尿病可能通过促进炎症部位破骨细胞生成,增强骨吸收,促进牙周炎骨丧失。Objective：To investigate the mechanism of periodontitis-associated bone loss in diabetic rats. Methods： Sixty-two six-week-old male SD rats were randomly divided into three groups： diabetic periodontitis group （DP） , periodontitis group（P） and normal control group（N）. Diabetic rats were induced by intraperitoneal injection of streptozotocin（STZ）. Periodontitis was initiated by ligating floss around maxillary second molars and oral inoculation with periodontal pathogen Porphyromonas gingivalis. Three or six weeks later, animals were sacrificed and the specimens were prepared for histological analysis. Assessment was carried out by using different visual and histologi- cal approaches. Visual assessment included alveolar bone loss（ABL）. Histological examination included osteoclast numbers in the inflammatory area. Statistical significance was determined by one-way ANOVA with significance set at P 〈 0.05. Results ： The sequence of mean alveolar bone loss among each group was group DP 〉 group P 〉 group N. Inter-group analysis revealed higher osteoclast numbers in the inflammatory area of group DP and group P when compared with group N （ P 〈 0.05 ）. Compared with group P, group DP had more higher osteoclast numbers （ P 〈 0.05 ）. Conclusion ： Diabetes can increase the severity of periodontitis. Accelerated alveolar bone loss was observed in diabetic rats. Increased osteoclast numbers in the inflammatory area in diabetes may contribute to the exaggerated destruction of alveolar bone. Diabetes may enhance periodontitis-associated bone loss by increasing the formation of osteoclasts in the periodontal tissue.

关 键 词：牙周炎 糖尿病 疾病模型 大鼠 

分 类 号：R781.4[医药卫生—口腔医学]