bFGF反义寡核苷酸对肾癌细胞凋亡的影响  

Effects of antisense oligodeoxynucleotide targeting bFGF on apoptosis of human renal carcinoma cell line

作　　者：吴宁[1] 胡德蓉[1] 齐洁琳[1] 李莉[2] 王新红[1] 蒋树丽[1] 李劲[1] 

机构地区：[1]山东省肿瘤防治研究院内一科,济南250117 [2]山东省济宁医学院附属医院检验科,济宁272100

出　　处：《肿瘤》2009年第1期42-45,共4页Tumor

摘　　要：目的:探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)反义寡核苷酸对肾癌细胞凋亡的影响,以期寻找肾癌反义治疗的合适药物。方法:以脂质体作为转染载体,将bFGF反义寡核苷酸转染人肾癌细胞系786-0,应用RT-PCR和Western印迹法检测转染bFGF反义寡核苷酸后肾癌细胞中bFGF mRNA及其蛋白的表达,应用流式细胞仪检测转染bFGF反义寡核苷酸后肾癌细胞的凋亡率。结果:与转染错义寡核苷酸组相比,转染bFGF反义寡核苷酸组786-0细胞中bFGF mRNA及其蛋白的表达有明显下降,且转染bFGF反义寡核苷酸可明显提高786-0细胞的凋亡率(P<0.01)。结论:经bFGF反义寡核苷酸作用后,肾癌细胞bFGF的表达受到了抑制,同时促进了肾癌细胞的凋亡,因此应用bFGF反义寡核苷酸治疗肾癌可能是一种较有希望的肿瘤治疗新方法。Objective： To investigate the effects of antisense oligodeoxynucleotide targeting basic fibroblast growth factor （bFGF） on apoptosis of human renal carcinoma cell line and find the appropriate agents for renal carcinoma therapy. Methods： The antisense bFGF oligodeoxy-nucleotides were transfeeted into human renal carcinoma cell line 786-0 by lipofeetin. Inhibition of bFGF mRNA and protein expressions was investigated by RT-PCR and Western blotting after transfection, respectively. The apoptosis of transfected cells was determined by flow cytometric analysis. Results： The bFGF mRNA and protein expressions were significantly decreased after transfection with antisense bFGF oligodeoxynucleotides compared with those transfeeted with missense bFGF oligodeoxy- nucleotides. Transfection with antisense bFGF oligodeoxynucleotides markedly increased the apoptotic rate of 786-0 cells （ P 〈0. 01 ）. Conclusion： Treatment with antisense bFGF oligodeoxynucleotides inhibited the expressions of bFGF at mRNA and protein levels and increased the apoptosis of renal carcinoma 786.0 cells, Antisense bFGF oligodeoxynucleotides may be a desirable novel approach for treatment of renal carcinoma.

关 键 词：癌 肾细胞 寡核苷酸类 反义 成纤维细胞生长因子 碱性 细胞凋亡 

分 类 号：R737.11[医药卫生—肿瘤]