血管紧张素Ⅱ及其受体阻滞剂对鼻咽癌细胞血管内皮生长因子的表达及其生物学行为的影响  

Effects of angiotensin Ⅱ and angiotensin Ⅱ type 1 receptor blocker on vascular endothelial growth factor expression and biological behavior of nasopharyngeal carcinoma cells

作　　者：伍钢[1] 王琼[1] 赵伟[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院肿瘤中心,武汉430023

出　　处：《肿瘤》2009年第1期49-52,共4页Tumor

摘　　要：目的:研究血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)及血管紧张素Ⅱ1型受体(angiotensinⅡ type 1 receptor,AT1R)阻滞剂缬沙坦对鼻咽癌细胞株CNE-2血管内皮生长因子(vascular endothelial growth factor,VEGF)表达及其生物学行为的影响。方法:应用RT-PCR和ELISA法分别检测干预前后CNE-2细胞VEGF基因和蛋白水平表达的变化。采用MTT法检测AngⅡ对CNE-2细胞增殖的影响。采用体外侵袭实验检测AngⅡ和缬沙坦对CNE-2细胞侵袭力的影响。结果:AngⅡ可诱导CNE-2细胞表达VEGF(P<0.05),并呈剂量依赖效应。AngⅡ可提高CNE-2细胞增殖率和侵袭能力(P<0.05),而缬沙坦可抑制AngⅡ的诱导作用。结论:AngⅡ可诱导鼻咽癌CNE-2细胞的增殖和侵袭,AT1R阻滞剂可抑制此种诱导作用,其机制可能涉及对VEGF的表达调控。Objective： To investigate the effects of angiotensin Ⅱ （Ang Ⅱ ） and Aug U type 1 receptor （AT1 R） blocker valsartan on vascular endothelial growth factor （VEGF） expression and biological behavior of nasopharyngeal carcinoma cells. Methods： VEGF expression and secretion in CNE-2 cells were determined by RT-PCR and ELISA, respectively, in vitro. Effects of Ang Ⅱ and AT1R blocker on the proliferation of nasopharyngeal carcinoma cells were evaluated by MTT assay in vitro and the influence of Ang Ⅱ and valsartan on the invasion capacity of cells CNE-2 was detected by 24-well Matrigel invasion test. Results： Ang Ⅱ dose-dependently induced the expression and secretion of VEGF in nasopharyngeal carcinoma cells in vitro and enhanced the proliferation and invasion capacity of CNE-2 cells （ Both P 〈 0.05 ）. AT1R blocker valsartan reversed the effect of Ang Ⅱ. Conclusion： Ang Ⅱ induced proliferation and invasion of nasopharyngeal carcinoma cell while AT1R blocker valsartan inhibited it. The underlying mechanism may be related with regulation of VEGF expression and secretion.

关 键 词：鼻咽肿瘤 血管紧张素Ⅱ 血管内皮生长因子 增殖 肿瘤侵犯 

分 类 号：R739.6[医药卫生—肿瘤]