E-选择素预处理对大鼠脑缺血-再灌注损伤的影响  

Effect of E-selectin pretreatment on cerebral ischemia-reperfusion injury in rats

作　　者：苗佳音[1] 杨磊[1] 梁庆成[2] 

机构地区：[1]哈尔滨医科大学附属第二临床医院神经内科,黑龙江哈尔滨150086 [2]华中科技大学同济医院神经内科,湖北武汉430030

出　　处：《中国病理生理杂志》2009年第1期107-111,共5页Chinese Journal of Pathophysiology

基　　金：黑龙江省教育厅科学技术研究资助项目(No.10551150)

摘　　要：目的:研究E-选择素鼻粘膜耐受对大鼠脑缺血-再灌注损伤的作用及其机制。方法:E-选择素或PBS单程诱导耐受或加强诱导耐受48 h后,用改良的Zea Longa线栓法制备大鼠大脑中动脉缺血模型,缺血2h再灌注22 h后,流式细胞仪测定血中CD3+CD4+T细胞及CD3+CD8+T细胞含量,TTC染色法测定脑梗死体积,RT-PCR检测脑梗死区E-选择素、细胞间黏附分子-1(ICAM-1)、淋巴细胞功能相关抗原-1(LFA-1)的表达,黄嘌呤氧化酶法检测脑组织中SOD水平。结果:E-选择素单程诱导耐受组CD3+CD4+T细胞与CD3+CD8+T细胞比值增加(P<0.05)。与其它组相比,E-选择素加强诱导耐受组脑梗死体积减小了40.87%(P<0.05),CD3+CD8+T细胞所占比例减小、CD3+CD4+T细胞与CD3+CD8+T细胞比值增高(P<0.05),脑组织中SOD水平升高(P<0.05),E-选择素、ICAM-1表达减少(P<0.05),LFA-1表达有减少趋势。结论:E-选择素鼻黏膜耐受诱导脑缺血耐受可减轻脑缺血-再灌注损伤,其作用机制与CD8+T细胞减少,CD4+T细胞与CD8+T细胞比值增加、SOD水平升高及E-选择素、ICAM-1表达减少有关。AIM ： To study the effect of nasal mucosal tolerance to E - selectin on cerebral ischemia - reperfusion injury. METHODS： Two different doses （ single and booster） of E - selectin or PBS were dropped into membrana mucosa nasi of rats. The middle cerebral artery occlusion （MCAO） model referring to Zea Longa method with modifications was performed 48 h after the last dose of E - selectin or PBS. After 2 h ischemia and 22 h reperfusion, the numbers of CD3^＋CD4^＋ T - lymphocyte and CD3^＋ CD8^＋ T lymphocyte subgroup in the blood were examined with flow cytometry. Rats were killed, then part of the animals was used to measure the cerebral infarction volume by TTC staining, mRNA expressions of E - selectin, ICAM - 1 and lymphocyte function - associated antigen - 1 （ LFA - 1 ） were determined by RT - PCR and activity of SOD was determined by xanthinoxidanse method in ischemic cortex of the other part of animals. RESULTS： The ratio of the numbers of CD3^＋CD4^＋ T - lymphocytes and CD3^＋ CD8^＋ T - lymphocytes increased in E - selectin single pretreatment group （P 〈 0. 05 ）. Compared to other groups, E - selectin booster pretreatment group showed decreased CD3^＋ CD8^＋T - lymphocytes （ P 〈 0. 05 ）, increased ratio of the numbers of CD3^＋CD4^＋ T - lymphocytes and CD3^＋ CD8^＋T - lymphocytes （P 〈 0. 05 ）, reduced cerebral infarction volume by 40.87% （ P 〈 0. 05 ）, heightened activity of SOD （ P 〈 0. 05 ）, lowed E - selectin mRNA and ICAM - 1 mRNA expression （ P 〈 0. 05 ）, and less tendency of LFA - 1 mRNA expression. CONCLUSION： E - selectin induces cerebral ischemic tolerance and relieves cerebral ischemia - reperfusion injury. The mechanisms are related to the changes in the ratio of CD4^＋ T - lymphocyte and CD8^＋ T - lymphocyte. The heightened activity of SOD, the lowed mRNA expressions of E - selectin and ICAM - 1, as well as the less tendency of LFA - 1 mRNA expression are also involved.

关 键 词：E-选择素 鼻黏膜耐受 脑缺血 再灌注损伤 CD3^+CD8^+T淋巴细胞 

分 类 号：R743[医药卫生—神经病学与精神病学]