白藜芦醇对纤维蛋白上调大鼠脑血管内皮细胞白细胞介素-6表达的影响  

The Effect of Resveratrol on Expression of Interleukin-6 Induced by Fibrin in Rat Brain Vascular Endothelial Cells

作　　者：薛峥[1] 程明珍[1] 胡晓晴[1] 张苏明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《医药导报》2009年第2期178-181,共4页Herald of Medicine

摘　　要：目的观察白藜芦醇(resveratrol,Res)对体外大鼠脑血管内皮细胞与纤维蛋白共培养高表达白细胞介素-6(interleukin-6,IL-6)转录及蛋白水平的影响。方法大鼠脑血管内皮细胞分离后培养,加入1.0 mg.mL-1纤维蛋白和不同浓度白藜芦醇,通过RT-PCR检测脑血管内皮细胞中IL-6转录水平,应用酶联免疫方法(ELISA)定量检测培养基中的IL-6水平。结果加入不同浓度(0,1,5,10,25和50μmol.L-1)的白藜芦醇24 h后,25和50μmol.L-1白藜芦醇组培养基中IL-6水平显著降低(均P<0.01);RT-PCR结果显示,25和50μmol.L-1白藜芦醇组大鼠脑血管内皮细胞中IL-6 mRNA显著下调(均P<0.01)。结论白藜芦醇可降低纤维蛋白导致的大鼠脑血管内皮细胞中IL-6的表达。Objective To investigate the effect of Resveratrol on expression of interleukin-6 （IL-6） induced by fibrin in rat brain vascular endothelial cells . Methods The concentration of IL-6 was measured by Elisa kit and the mRNA of IL-6 was measured by RT-PCR after cultured with 1.0 mg· mL^-1 fibrin polymerization on rat brain vascular endothelial cells in the presence or absence of different concentration of resveratrol （0 -50 μmol·L^-1 ）. Results After 24 h of incubation,25 and 50 μmol· L^-1 of resveratrol could significantly reduce IL-6 protein expression（P 〈 0.01 ）and IL-6 mRNA level （P 〈 0.01 ） induced by fibrin compared to the control group. Conclusion Resveratrol is potent inflammatory cytokiue inhibitor which could reduce IL-6 expression in the co-cuhure of rat brain vascular endothelial cells with fibrin.

关 键 词：白藜芦醇 纤维蛋白 脑血管内皮细胞 白细胞介素-6 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R286[医药卫生—临床医学]