分子信标探针荧光法测定慢性粒细胞性白血病相关基因  

Molecular-beacon-based fluorometric assay for detection of short DNA species of chronic myelogenous leukemia

作　　者：王昆[1] 陈敬华[1] 庄茜[1] 罗红斌[1] 李光文[1] 林新华[1] 

机构地区：[1]福建医科大学药学院药物分析系,福州350004

出　　处：《分析试验室》2009年第2期5-7,共3页Chinese Journal of Analysis Laboratory

基　　金：国家863计划(2006AA02Z4Z1);国家自然科学基金(20675015);福建省自然科学基金(2006I0016);福建省教育厅基金(2005K051)项目资助

摘　　要：根据慢性粒细胞性白血病(CML)相关基因b3a2序列设计了一种带有荧光基团和淬灭基团的凸环结构探针(分子信标,MB),研究其与互补目标DNA杂交前后的荧光变化行为,建立了b3a2基因检测的新方法。在最适条件下,得到杂交后溶液荧光强度与本底荧光强度的比值(S/B)和目标DNA的浓度呈线性关系,r=0.9973,线性范围4.0×10-9～3.2×10-8mol/L。该方法为实际样品的检测奠定了基础。To study the change of fluorimetric behavior of a DNA probe after hybridization, a combination of DNA hairpin structure with a pair of fluorophore and quencher （molecular beacon, MB） was designed according to the DNA sequences relating to Chronic Myelogenous Leukemia （CML, Type b3a2） and a novel method for the determination of type b3a2 gene was established. Under optimal conditions, the relationship between the ratio of signal to background （S/B） and the concentration of DNA （C） was linear in the range of 4 × 10^-9 - 3.2 × 10^-8 mol/L with a correlation coefficient of 0.9973. These results indicated this approach had rapid determination, high sensitivity, high specificity and good selectivity, and it could be applied to the detection of CML in the real samples for medical diagnostics.

关 键 词：慢性粒细胞性白血病 分子信标 荧光检测分光光度法 

分 类 号：O657.3[理学—分析化学]