纳米复合物修饰电极的电化学传感器检测芦丁  

Determination of rutin by a nanocomposite modified electrochemical sensor

作　　者：孔令蜜[1] 李梦瑶[1] 彭斌[1] 朱佩思[1] 黄杉生[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《分析试验室》2009年第2期8-11,共4页Chinese Journal of Analysis Laboratory

基　　金：国家高技术研究发展计划(863计划)课题(2007AA06Z402);上海市科委创新行动计划(0852010400)项目资助

摘　　要：研制了纳米复合物修饰电极,碳纳米管与表面含有大量氨基的壳聚糖在玻碳电极表面首先形成碳纳米管/壳聚糖膜,通过膜表面丰富的氨基与纳米Au的强静电吸附,在玻碳电极表面获得均匀致密的纳米金修饰层。这种基于纳米复合材料制备的新型电化学传感器对芦丁具有很好的响应,可以快速地实现电极与芦丁之间的直接电子转移,有良好的稳定性。芦丁的测定线性范围为4.00×10-7～1.77×10-5mol/L,最低检测限为1.29×10-7mol/L。由于抗坏血酸在该修饰电极上的氧化电位出现显著负移,因此可避免抗坏血酸对芦丁测定的干扰。该方法可以不经预分离直接检测药物中的芦丁含量。A new electrochemical sensor for the determination of rutin was prepared. A carbon nanotube/chitosan layer with positive charge was first coated on a glassy carbon electrode, then an Au nanoparticle layer with negative charge was strongly adsorbed on the surface of the electrode. This biosensor, with good stability, displays the current response to rutin due to the direct electron transfer between rutin and the carbon nanotube/chitosan/Au nanoparticle interface electrode. The biosensor can be used for the determination of rutin in the range of 4.00 × 10^-7 - 1.77 ×10^-5 mol/L with the detection limit of 1.29 ×10^-7 moL/L. Compared with the oxidation of AA at the bare GC electrode, the oxidation potential of AA at the modified electrode negatively shifted 200 mV. The interference of AA in the determination of rutin can be neglected. The method has been used for the determination of rutin in medicament without separation.

关 键 词：碳纳米管 方波伏安法 芦丁 纳米金 抗坏血酸 

分 类 号：O557.1[理学—热学与物质分子运动论]