重组人TNF介导白血病细胞程序性死亡及其临床意义  

The study of programmed cell death induced by rhTNF and its clinical significance

作　　者：李龙[1] 罗绍凯[1] 张国材[1] 潘辉[1] 刘亚光[1] 陈永雄[1] 尹培达[1] 

机构地区：[1]中山医科大学第一附属医院血液科

出　　处：《癌症》1998年第3期175-177,共3页Chinese Journal of Cancer

摘　　要：目的：为探讨ＴＮＦ诱导白血病细胞程序性死亡的发生机制。方法：应用体外细胞毒性试验，电镜及流式细胞仪进行检测和观察。结果：ＴＮＦ可诱导白血病细胞程序性死亡和杀伤白血病细胞，随着其浓度增加，凋亡细胞数也增加。结论：ＴＮＦ介导白血病细胞程序性死亡在白血病患者的治疗中有重要价值。Purpose: To investigate the mechanism by which rhTNF induces programmed cell death. Methods: MTT was performed for cytotoxic activity of TNF against leukemic cell in vitro. Electronmicroscopy and flow cytometry were performed for TNF-induced apoptosis. Results: It was found that TNF could induce programmed cell death of K562 cell and kill K562 cell. With increasing concentration of rhTNF, the number of apoptotic cells increased. Conclusions: These results suggest that apoptosis induced by TNF plays an important role in the therapy of leukemia

关 键 词：肿瘤坏死因子 程序性死亡 白血病细胞株 

分 类 号：R733.705[医药卫生—肿瘤]