半边旗抗肿瘤有效成分6F对HL-60细胞周期的影响及体外增效作用  

The effect of a diterpenoid isolated from pteris semipinnata on the HL-60 cell cycle and its enhanced in vitro cytotoxicity by anticancer drugs

作　　者：何承伟[1] 梁念慈[1] 莫丽儿[1] 张晓[1] 李金华[1] 

机构地区：[1]广东医学院医用生化研究所

出　　处：《癌症》1998年第3期191-193,共3页Chinese Journal of Cancer

基　　金：广东省高教局重点学科专款资助

摘　　要：目的：研究半边旗抗肿瘤有效成分６Ｆ对ＨＬ－６０细胞周期的影响及对常用抗肿瘤药的体外增效作用。方法：应用流式细胞光度术（ＦＣＭ）测定细胞周期，应用噻唑蓝（ＭＴＴ）法测定药物对细胞的抑制率。结果：不同浓度６Ｆ作用６小时即可使ＨＬ－６０细胞Ｓ期及Ｇ２／Ｍ期比例升高，Ｇ１期比例下降，并呈一定的剂量效应关系，当作用到２４小时后，Ｓ期比例进一步升高，但Ｇ２／Ｍ期比例稍有回落。低浓度６Ｆ分别与２－氯代脱氧腺苷（２－ＣＬｄＡｄｏ），顺铂（ＣＤＤＰ），长春新碱（ＶＣＲ），氟尿嘧啶（５ＦＵ）合用可增强它们对ＨＬ－６０细胞的杀伤作用，ｑ值大于０．８５，与各药有相加或协同作用，６Ｆ对２－ＣｌｄＡｄｏ，ＣＤＤＰ，ＶＣＲ，５ＦＵ的增效倍数分别为１．５８，１．５３，１．５５，１．３８。结论：６Ｆ可明显阻断ＨＬ－６０细胞在Ｓ期及Ｇ２／Ｍ期；６Ｆ可增强上述药物对ＨＬ－６０细胞的杀伤作用。已知，２－ＣｌｄＡｄｏ阻断细胞在Ｓ期，ＣＤＤＰ和ＶＣＲ阻断Ｇ２／Ｍ期，５ＦＵ阻断Ｇ１期。鉴于所试药物对细胞周期的影响不同，提示６Ｆ的体外增效作用可能与此有关。Objective: To observe the effect of 6F, one of the diterpenoids isolated from Pteris semipinnata L. (PsL), on the HL-60 cell cycle kinetics and its enhanced cytotoxicity when combined with other antitumor agents. Methods: Cell cycle was determined using F1ow Cytometry (FCM) analysis, Cytotoxicity assay was tested by MTT method. Results: The FCM data showed that the distribution of HL-60 cells among phases of the cell cycle was changed when treated with 6F. After exposure of the cells to various concentration of 6F for 6h, the S and G2/M phase ratio was increased significantly in a dose-dependent manner. After 24h of exposure, S hpase ratio increased further. The inhibitory rates of 6F combined with 2-CldAdo or CDDP or VCR or 5FU were higher than that of these drugs used alone, q values were more than 0.85. Their enhancement times by 6F were 1.58, 1.53, 1.55, 1.38, respectively. Conclusion: The results indicated that 6F could arrest HL-60 cells in S and G2/M phase, 6F could enhanced the cytotoxicity of the drugs mentioned above. it is known that 2-CldAdo arrests cells in S phase, CDDP and VCR arrest cells in G2/M phase, 5FU arrests cells in G1 phase. Thr different effect of these agents on the various phases of cell cycle kinetics may be partially explained the enhanced effect of 6F.

关 键 词：半边旗 药理学 6F HL-60 细胞周期 肿瘤 

分 类 号：R730.53[医药卫生—肿瘤] R931.71[医药卫生—临床医学]