肾细胞癌γ-谷氨酰转移酶糖链的凝集素分析  

ANALYSIS OF SUGAR CHAIN STRUCTURE OF γ-GLUTAMYLTRANSPEPTIDASE IN RENAL CELL CARCINOMA USING LECTIN AFFINITY CHROMATOGRAPHY

作　　者：朱同玉[1,2] 张元芳[1,2] 张永康 章仁安[1,2] 唐群业[1,2] 邹秋锋[1,2] 

机构地区：[1]上海医科大学中山医院泌尿外科 [2]华山医院泌尿外科

出　　处：《上海医科大学学报》1998年第3期193-196,共4页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金;国家教委博士点专项基金

摘　　要：目的探讨肾细胞癌标记物。方法用荧光分光光度法测定了正常肾皮质及肾细胞癌组织中γ－谷氨酰转移酶（γ－ＧＴ）的活力，并用凝集素亲和层析法测定了γ－ＧＴ的糖链与ＣｏｎＡ吸附百分比以研究肾细胞癌γ－ＧＴ中糖链的结构变化。结果肾细胞癌组织中的γ－ＧＴ均较正常肾皮质γ－ＧＴ活力明显降低。组织γ－ＧＴ糖链ＣｏｎＡ分析，肾癌组与正常肾皮质组相比，不吸附组分由（６８．２±１０．６）％降低至（３９．３±１０．８）％，而弱吸附和强吸附组分均显著升高，其中弱吸附部分由（２２．１±９．３）％增加至（４０．８±７．９）％，强吸附部分由（９．７±５．４）％增加至（１９．９±８．５）％；尿液γ－ＧＴＣｏｎＡ分析，与对照组相比，肾癌组不吸附组分由（６６．９±８．０）％降低至（５７．８±１１．２）％，差别有显著意义，弱结合组分别为（２７．８±８．３）％和（２４．１±８．７）％，两者无明显差别；强结合组由（９．０±５．８）％增加至（１４．４±８．９）％，差别有显著意义。结论肾细胞癌组织中γ－ＧＴ活力明显降低，肾癌组织及尿液γ－ＧＴ的糖链结构也发生明显的变化，尿液γ－ＧＴＣｏｎＡ层析可用于肾癌的诊断。URPOSE To study the activity and sugar chain structure of γ-glutamyltranspeptidase (γ-GT) in renal cell carcinoma (RCC) in order to find a tumor marker of RCC.METHODS The specific activity of γ-GT was determinated by using fluorimetric assay and the sugar chain structure of it was analyzed with Con A affinity chromatography in samples from the normal renal cortex, RCC tissues and urine.RESULTS Compared with the normal renal cortex, in RCC, the specific activity of γ-GT dec￣reased significantly in all samples. The structure of sugar chain in tissue and urine also be changed. The unbound fraction (Con A-) of γ-GT from Con A affinity chromatography decreased from (68.2±10.6)% to (39.3±10.8)%, weakly (Con A+) and strongly (Con A) bound fraction elevated from (22.1±9.3)% to (40.8±7.9)% and (9.7±5.4)% to (19.9±8.5)% respectively. Compared with the normal urine, in RCC, Con A- decreased from (66.9±8.0)% to (57.8±11.2)%, Con A increaced from (9.0±5.8)% to (14.4±8.9)%. The difference between normal subjects and RCC patients were statistically significant.CONCLUSIONS Con A chromatography of urine γ-GT may be used in the diagnosis of RCC.

关 键 词：肾细胞癌 Γ谷氨酰转移酶 凝集素 糖链 

分 类 号：R737.11[医药卫生—肿瘤]