大鼠损伤皮肤组织5-羟色胺含量测定  

Quantitive Analysis of 5 hydroxytryptamine Levels on Edges of Incised Skin of Rats

作　　者：赖跃 廖志钢 刘敏 吴家马 

出　　处：《华西医科大学学报》1998年第2期189-191,共3页Journal of West China University of Medical Sciences

摘　　要：为寻找生前损伤早期诊断指标，采用免疫散射浊度分析法，对生前、死后不同时间形成的大鼠切创皮肤的５ＨＴ进行对比分析。结果显示：生前损伤５分钟，５ＨＴ含量明显升高，１５分钟达高峰，６０分钟开始下降。生前损伤各组５ＨＴ含量均较自身对照组明显升高（升高４０％以上，Ｐ＜００５）；而死后５分钟和１５分钟组皮肤中５ＨＴ含量均较自身对照组升高（Ｐ＜００５），但增加幅度均低于３６％。提示，如创缘皮肤５ＨＴ含量比自身对照升高４０％。To find index for early diagnosis of antemortem wound,the authors used the ICS analysis in a quantitive study of 5 hydroxytryptamine(5 HT) levels on rat's incised skin antemortem wound in comparison with the postmortem wound inficted at various intervals.The results showed that the 5 HT increased significantly at 5 min,reached a peak at 15 min and decreased at 60 min on antemortem wounds.The 5 HT levels of antemortem groups were prominently high(more than 40%, P <0.05) as compared with those of postmortem group and the levels of 5 HT on the wounds of postmortem groups were higher (35 77%, P <0.05) than those of controls as well.The authors suggest that in the cases where the levels of 5 HT are as high as 40% on the edges of the injured skin,the injury should be considered an antemortem wound.

关 键 词：免疫组织化学 5-羟色胺 生前伤 尸检 

分 类 号：R361.1[医药卫生—病理学] D919.4[医药卫生—基础医学]