萘对斑马鱼(Danio rerio)内脏团抗氧化防御系统的胁迫与生物响应  

Stress and Biological Response of Naphthalene on the Antioxidant Defense System in Visceral Mass of Zebrafish (Danio rerio)

作　　者：王隽媛[1] 边红枫[1] 金香琴[1] 盛连喜[1] 

机构地区：[1]东北师范大学城市与环境科学学院,国家环境保护湿地生态与植被恢复重点实验室,长春130024

出　　处：《环境科学》2009年第2期516-521,共6页Environmental Science

基　　金：国家重点基础研究发展规划(973)项目(2004CB418505);吉林省科技发展计划项目(20065021)

摘　　要：经急性毒性实验,得到萘对成体斑马鱼96h的半数致死浓度(LC50)为11.8mg.L-1.在此基础上,设置5个浓度梯度:0、1/6LC50、1/4LC50、1/3LC50、1/2LC50,研究了在不同的暴露时间下(0.5、1、2、4、、14d),萘对斑马鱼抗氧化防御系统的影响.结果表明,还原型谷胱甘肽(glutathione,GSH)、谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)和谷胱甘肽硫转移酶(glutathioneS-transferase,GST)对萘非常敏感,在暴露0.5d时就受到抑制或诱导.0.5d后GPx活性整体上被诱导,只在第14d时高浓度组(1/3LC50和1/2LC50浓度组)GPx活性被抑制;GST和GSH则总体上是低于对照的.超氧化物岐化酶(superoxide dismutase,SOD)活性在2d后呈现先诱导后抑制;过氧化氢酶(catalase,CAT)活性在1d后整体上受抑制.萘对斑马鱼内脏团抗氧化防御系统能产生影响,其变化可作为生物标志物,来评价暴露于多环芳烃有机污染的鱼类的生物学效应.With the acute toxicity test, we obtained the median lethal concentration （ LC50 ） of naphthalene to the zebrafish for 96 h, which was 11.8 mg· L^-1 . Then we set up five treatments（0, 1/6 LC50, 1/4 LC50, 1/3 LC50 and 1/2 LC50 ） and exposed zebrafish to these treatments for 0.5, 1, 2, 4, 7 and 14d respectively to study the effects of naphthalene on the antioxidant defense system in visceral mass of zebrafish. The results showed that GSH, GPx and GST were very sensitive to naphthalene and were inhibited or induced at 0.5 d exposure. The activities of GPx were induced on the whole after 0.5 d, but were inhibited at higher concentration treatments（1/3 LC50 and 1/2 LC50 treatments） at 14d exposure; while the activities of GST and the contents of GSH were almost lower than that of the control. The activities of SOD were induced first and then inhibited after 2d; the activities of CAT were inhibited almost in the all treatments after ld. The effects of naphthalene on the antioxidant defense system in visceral mass of zebrafish could be used as biomarkers to estimate the biological effect of fish exposed to polycyclic aromatic hydrocarbons.

关 键 词：萘 斑马鱼(Danio rerio) 急性毒性 抗氧化防御系统 

分 类 号：X171.5[环境科学与工程—环境科学]