人参皂苷化学修饰物PM1诱导肝癌HepG2细胞凋亡  

Apoptosis of hepatocellular carcinoma HepG2 cells induced by ginsenoside modifier PM1

作　　者：杨春光[1] 弓晓杰[1] 孙长滨[1] 

机构地区：[1]大连大学医学院,辽宁大连116622

出　　处：《中国医药导报》2009年第3期21-22,共2页China Medical Herald

基　　金：辽宁省教育厅科研基金项目(No.20060070)

摘　　要：目的:探讨人参皂苷化学修饰物PM1体外诱导肝癌HepG2细胞凋亡作用。方法:采用四甲基偶氮唑蓝(MTT)还原法检测不同浓度的人参皂苷化学修饰物PM1对肝癌HepG2细胞增殖的作用;流式细胞仪分析肿瘤细胞凋亡周期及细胞凋亡率。结果:PM1抑制肝癌HepG2细胞生长,呈浓度依赖关系。用50和100mg/LPM1处理细胞24h后,PM1诱导了细胞凋亡和调节细胞周期,凋亡率与对照组比较明显升高(P<0.01)。结论:PM1抑制肝癌HepG2细胞生长,其机制可能是通过诱导凋亡来发挥作用的。Objective： To study the effect of PM1 on inducing apoptosis of HepG2 ceils in vitro. Methods： The HepG2 ceils were treated with different doses of PM1. Cell proliferation was measured by MTT assay. Cell cycle and apoptosis rate were analyzed by flow cytometry. Results： PM1 inhibited proliferation of HepG2 ceils in concentration-dependent manner. FCM assay showed that PM1 regulated cell cycle and induced apoptosis of HepG2 cells after treated with 50 and 100 mg/L PM1 for 24 h. The apoptosis rates were increased （P〈0.01）. Conclusion： PM1 probably inhibits the proliferation of HepG2 cells by inducing apoptosis.

关 键 词：人参皂苷化学修饰物 肝癌 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]