金雀异黄酮对鸡骨骼肌卫星细胞脂质过氧化和抗氧化酶活性的影响  

Effects of Genistein on Lipid Peroxidation and Antioxidant Defense System in Chick Skeletal Muscle Cells

作　　者：蒋守群[1] 蒋宗勇[1] 郑春田[1] 马现永[1] 席鹏彬[1] 

机构地区：[1]广东省农业科学院畜牧研究所广东省动物育种与营养公共实验室,广东广州510640

出　　处：《中国畜牧杂志》2009年第1期38-42,共5页Chinese Journal of Animal Science

基　　金：广东省自然基金团队项目(05200576)

摘　　要：为了探明大豆异黄酮主要成分金雀异黄酮(GEN)对鸡骨骼肌卫星细胞的抗氧化保护效应,试验研究了GEN对离体培养的鸡骨骼肌卫星细胞脂质过氧化、抗氧化酶活性和基因表达的影响。试验采用岭南黄羽肉鸡骨骼肌卫星细胞接受不同浓度的GEN处理48h后,测定细胞培养上清液中谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶活性以及丙二醛含量,应用TaqMan实时荧光定量PCR法测定GEN对骨骼肌卫星细胞中谷胱甘肽过氧化物酶基因表达的影响。结果表明:与空白对照组相比,添加金雀异黄酮组骨骼肌卫星细胞培养上清液中丙二醛含量显著降低(P<0.05),添加1μmol/L和16μmol/L金雀异黄酮显著提高了鸡骨骼肌卫星细胞培养上清液中超氧化物歧化酶活性(P<0.05),添加4μmol/L和16μmol/L金雀异黄酮显著提高了谷胱甘肽过氧化物酶活性(P<0.05),细胞培养液中添加金雀异黄酮具有提高谷胱甘肽过氧化物酶mRNA拷贝数的趋势(P>0.05)。To investigate the protective effect of genistein on antioxidative defence system and lipid peroxidation in chick skeletal muscle cells, chick skeletal muscle cells were treated with 0, 1, 4, 16 umol/L genistein. The activities of glutathione peroxidase（GSHPx）, superoxide dismutase（SOD） and catalase（CAT） and the concentration of malondialdehyde （MDA） were measured in supernatant of the medium for culture of chick skeletal muscle cells. Meanwhile the expression of GSHPx was determined by RT-PCR method. Compared to control group, treatmed with 1, 4 or 16 umol/L genistein significantly decreased MDA production by 33.85%（P 〈 0.05）, 33.08%（P 〈 0.05）, and 50.77%（P 〈 0.05）, respectively. The activity of SOD was significantly increased after treated with 1 p.mol/L or 16umol/L genistein （P 〈 0.05）. The addition of genistein at 4 or 16 umol/L improved the activity of GSHPx （P 〈 0.05）. In conclusion, genistein could inhibit lipid peroxidation and improve antioxidative property by decreasing MDA production and increasing the activities of antioxidant enzymes in chick skeletal muscle cells.

关 键 词：金雀异黄酮 丙二醛 谷胱甘肽过氧化物酶 超氧化物歧化酶 骨骼肌卫星细胞 鸡 

分 类 号：S831.5[农业科学—畜牧学]