海带多糖抗血栓作用的量效观察  

DOSE-EFFECT STUDY OF POLYSACCHARIDE EXTRACTED FROM LAMINARIA JAPONICA ARESCH ON THROMBUS FORMATION

作　　者：葛邕[1] 王丽萍[2] 谢露[2] 陈蒙华[3] 

机构地区：[1]广西医科大学护理学院,南宁530021 [2]广西医科大学生理学教研室 [3]广西医科大学第一附属医院心血管病研究所

出　　处：《广西医科大学学报》2008年第6期829-831,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助课题(No.30660229);广西自然科学基金资助课题(No.桂科自0229033);广西医疗卫生资助项目(No.桂卫重200632;Z2006057)

摘　　要：目的:探索海带多糖体内外抗血栓作用的量效关系。方法:在体外实验中,将小鼠随机分为生理盐水组、肝素4个等级剂量组、海带多糖4个等级剂量组。体外旋转法观测血栓重量,并进行各组比较。在体内实验中,将大鼠随机分为生理盐水组、阿司匹林高低剂量组、海带多糖高低剂量组、肾上腺素模型组。注射肾上腺素制备内皮损伤大鼠模型,通过动-静脉环路法测定血栓湿重。结果:体外血栓形成实验表明,与生理盐水组比较,肝素和海带多糖各剂量组均显示出抗血栓形成作用(P<0.01),且作用随剂量的增大而增强。体内血栓形成实验表明,与模型组比较,海带多糖高剂量组、阿司匹林高低剂量组均能降低大鼠血栓湿重(P<0.01),显现出剂量依赖性。结论:海带多糖具有剂量依赖性的抗血栓形成作用。Objective： To explore the dose-effect of Polysaccharide extracted from Laminaria japonica Aresch （PL）. Methods： For experiment in vitro, the mice were randomly divided into saline group, four dose-grade heparin groups, four-dose-grade PL groups. A circumrotating method in vitro was used to measure the weight of thrombus and compared with each other. For experiment in vivo, rats were divided into saline group, high-dose and low0-dose aspirin groups, high-dose and low-dose PL groups and adrenaline model group. A rat model of endothelial injury was established via injecting adrenaline. An artery-vein loop was used to measure the weight of thrombus. Result： Compared with the saline group, all dose-grade groups of heparin and PL showed antithrombotic effect in vitro（P〈0.01）, which potentiate, the following dose in- crease. Compared with the adrenaline model group, high-dose PL group, high-dose and low-close aspirin groups decreased the weight of thrombus in vivo（P〈0.01）, with a dose-dependent relation. Conclusion： PL exhibited an antithrombotic effect in dose-dependent.

关 键 词：海带多糖 肝素 阿司匹林 血栓 

分 类 号：R965[医药卫生—药理学]