基因芯片检测健侧颈7移位术治疗小鼠全臂丛神经损伤后脊髓基因的表达变化  

Gene expression profiling of mice spinal cord after contralateral C_7 nerve root transfer by microarray analysis

作　　者：肇晖[1] 李继峰 罗鹏波[2] 顾玉东[2] 冯勇[2] 

机构地区：[1]复旦大学上海医学院中西医结合系,上海200032 [2]复旦大学附属华山医院手外科,上海200040

出　　处：《复旦学报（医学版）》2009年第1期1-9,共9页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金项目(3077218)

摘　　要：目的检测健侧颈7神经根移位术后脊髓基因表达的变化,探讨神经根移位术后脊髓可塑性形成的机制。方法30只雄性C57BC/6小鼠,体重20 g,随机分为实验组与对照组,每组15只。实验组又分为3个亚组:损伤即刻修复3个月组,损伤3个月、修复3个月组,损伤1个月、修复6个月组,每组5只。按照上述分组在制作全臂丛损伤模型后行健侧颈7移位术,于不同时间点取材应用Biostar M-140s基因芯片检测小鼠脊髓组织基因表达的变化,并与对照组进行比较。结果损伤即刻修复3个月组,损伤3个月、修复3个月组,损伤1个月、修复6个月组脊髓组织分别有55,6和118个基因发生表达水平的改变。其中编码免疫球蛋白重链、chitinase 3的基因在损伤即刻修复3个月组和损伤1个月、修复6个月组均表达增加。编码血管紧张素、collagen、myosin的基因在损伤即刻修复3个月组表达减少,而在损伤1个月、修复6个月组表达增加。突触蛋白synaptotagmin和synaptobrevin在损伤3个月、修复3个月组基因表达改变不明显,而在损伤1个月、修复6个月组表达明显升高。结论健侧颈7神经根移位术能引起脊髓组织基因表达水平发生变化,这种变化呈时间依赖性。免疫应答发生于术后各个阶段,而突触可塑性的形成主要发生在术后6个月。Objective To explore gene expression profile of the spinal cord after contralateral C7 nerve root transfer, as well as the mechanism of neural plasticity. Methods Thirty male C57BC/6 mice were randomly divided into experiment group and control group with 15 in each. Experiment group was randomly divided into 3 subgroups with 5 in each, which is subgroup 1 （three months after eontralateral C7 nerve root transfer treating immediate brachial plexus root avulsion）, subgroup 2 （three months after contralateral C7 nerve root transfer treating 3 months brachial plexus root avulsion）, and subgroup 3 （one month after contralateral C7 nerve root transfer treating 6 months brachial plexus root avulsion）, respectively. A model of contrallateral C7 nerve root transfer treating left total brachial plexus root avulsion was established in experiment group. We took advantage of BiostarM-140s microarray analysis to detect the differential expression of genes in spinal cord after contralateral C7 nerve root transfer. Results Contralateral C7 nerve root transfer could lead to changes in gene expression in spinal cord. There were 55, 6 and 118 differential expressed genes in subgroup 1, 2, 3 respectively. Among them, genes encoding for immunoglobulin heavy chain, chitinase 3 were decreased in sungroup 1 or 3. Genes encoding for angiopoietin, collagen and myotin were deceased in subgroup 1 while increased in subgroup 3. Intriguingly, synaptic proteins like synaptotagmin and synaptobrevin were up-regulated in subgroup 3, whereas no significant changes were found in sungroup 1 and 2. Conclusions The data indicate that changes in gene expression in spinal cord are induced by C7 nerve root transfer, the change displayed is time-dependent. Immune response is implicated in the whole stage of post-nerve root transfer. Synaptic plasticity occurrs mainly in 6 months.

关 键 词：臂丛神经损伤 神经移位 颈7神经根 基因芯片 基因表达 

分 类 号：R745.4[医药卫生—神经病学与精神病学] Q786[医药卫生—临床医学]