鼻咽癌中环氧化酶-2和潜伏膜蛋白-1的表达和相互关系的初步研究  

The expression and relationship of cyclooxygenase-2 and latent membrane protein-1 in nasopharyngeal carcinoma

作　　者：白薇琦[1] 汤建国[2] 

机构地区：[1]宁波市第二医院耳鼻咽喉科,浙江宁波315010 [2]浙江大学医学院附属邵逸夫医院耳鼻咽喉-头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2009年第3期105-108,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨鼻咽癌组织中的环氧化酶-2(COX-2)、潜伏膜蛋白-1(LMP1)的表达情况,同时分析了两者之间的关系。方法:53例鼻咽癌活检标本,8例鼻咽部慢性炎症常规石蜡切片标本作为对照组,用SP免疫组织化学法检测COX-2、LMP1的表达,采用SPSS12.0统计软件对实验数据进行统计。结果:在鼻咽癌组织中,COX-2阳性表达率为71.70%(38/53);LMP1阳性表达率为66.04%(35/53)。COX-2、LMP1的表达在颈部淋巴结转移组中均明显高于无颈部淋巴结转移组(P<0.05),但在年龄、性别、病理类型、临床分期的分组中均差异无统计学意义(均P>0.05)。在鼻咽癌组织中,LMP1和COX-2的表达存在正相关(γ=0.797,P<0.01)。结论:COX-2、LMP1在鼻咽癌组织中均有表达。COX-2、LMP1在鼻咽癌中的表达和鼻咽癌颈淋巴结转移存在显著相关性,但和年龄、性别、病理类型、临床分期无相关性。COX-2和LMP1在鼻咽癌组织中的表达呈正相关。LMP1可能通过多途径上调COX-2的表达,从而促进鼻咽癌颈部淋巴结转移。Objective： To discuss the expression and correlation of COX2 and LMP1 in NPC. Method： Fiftythree nasopharyngeal biopsy specimens of NPC patients who had been diagnosed definitely with pathology in our department from 2000 to 2005, and 8 nasopharyngeal biopsy specimens of chronic nasopharyngitis patients were collected. The expression of COX2,LMP1 were detected with streptavidin peroxidase immunohistoehemistry stai ning. All the datas were analyzed with SPSS 12.0. Result： The positive expression rate of COX2 was 71.70% （38/ 53） ,and that of LMP1 was 66.04% （35/53） of Nasopharyngeal carcinoma tissue. The higher expression rate was detected in neck lymph nodes metastasis group of nasopharyngeal earcinoma（P〈0.05）. While the expression rate of COX2, LMP1 was no significant relation with age, gender, clinical stage and pathological classification（P〈 0.05）. The expression of COX2 and LMP1 showed positive correlation （γ = 0. 797, P〈 0.01）. Conclusion： COX2 and LMP1 were highly expressed in nasopharyngeal carcinoma cells. The expressions of COX2 and LMP1 was sig nificantly associated with neck lymph nodes metastasis, and was none-significant relation with age, gender, clinical stage and pathological classification. The expression of LMP1 showed a significant positive correlation with that of COX2. LMP1 could enhance NPC neck lymph nodes metastasis by up-regulating the expression of COX2.

关 键 词：鼻咽肿瘤 环氧化酶-2 EB病毒 潜伏膜蛋白1 

分 类 号：R739.65[医药卫生—肿瘤]