血管细胞黏附分子-1及P-选择素在喉咽癌中的表达及与微血管密度的相关性研究  

The expression and relationship of vascular cell adhesion molecule-1 and P-selectin and microvessel density in hypopharyngeal carcinoma

作　　者：梁大鹏[1,2] 潘新良[1] 孙文海[2] 黄沂传[2] 

机构地区：[1]山东大学齐鲁医院耳鼻咽喉头颈外科,济南250012 [2]青岛大学医学院附属医院耳鼻咽喉头颈外科,山东青岛266003

出　　处：《临床耳鼻咽喉头颈外科杂志》2009年第3期115-118,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:检测喉咽癌组织中血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)及P-选择素的表达及其与微血管密度(microvessel density,MVD)间的关系,探讨VCAM-1及P-选择素在喉咽癌血管生成中的意义。方法:用免疫组织化学方法检测40例喉咽癌组织和10例正常口咽部黏膜组织中VCAM-1、P-选择素及CD34表达并计数MVD。结果:VCAM-1及P-选择素在喉咽癌组织中的表达率显著高于正常口咽部黏膜组织(P<0.01);喉咽癌组织的MVD值显著高于正常口咽部黏膜组织(P<0.01),MVD值与有无淋巴结转移密切相关(P<0.01);VCAM-1及P-选择素表达阳性组MVD值显著高于表达阴性组(P<0.01),且VCAM-1及P-选择素表达均与MVD呈正相关(P<0.05);在喉咽癌淋巴结转移组中,VCAM-1及P-选择素的表达呈正相关关系。结论:VCAM-1及P-选择素在喉咽癌组织中的高表达在肿瘤的浸润和转移中起重要作用,并与肿瘤血管生成有关。Objective： To investigate the expression and significance of vascular cell adhesion molecule-1 （VCAM-1） and P-selectin in hypopharyngeal carcinoma, and explore the relationship of VCAM-1 ,P-selectin and microvessel density （MVD）. Method：Expression of VCAM-1 and P selectin were detected by immunohistochemis try staining in 40 specimens of hypopharyngeal carcinoma and 10 specimens of normal mucosa of oral pharynx. MVD was assessed based on the expression of CD34. Result： VCAM-1 and P-selectin were detected in 26 out of 40 （65% ） and 29 out of 40 （72.5%） respectively in hypopharyngeal carcinoma, but none that in normal mucosa（P〈 0.01）. MVD in hypopharyngeal carcinoma was higher than that in normal mueosa and it was related to lymph node metastasis. MVD level was significantly higher in VCAM-1 and P-selectin-positive specimens than in negative ones（P〈0.01）,which also positively correlated with the expression of VCAM-1 and P selectin（P〈0.01）. The expression of P-selectin positively correlated with VCAM-1 in hypopharyngeal carcinoma specimens with lymph node metastasis. Conclusion：The higher expression of VCAM-1 and P-selectin play key roles in the invasion and metastasis of hypopharyngeal carcinoma and were correlated with vascularization.

关 键 词：喉咽肿瘤 血管细胞黏附分子-1 P-选择素 微血管密度 

分 类 号：R739.65[医药卫生—肿瘤]