壳寡糖的薄层层析分析  

TLC Analysis of Chitooligosaccharides

作　　者：陈小娥[1] 方旭波[1] 余辉[1] 廖智[2] 

机构地区：[1]浙江海洋学院食品与药学学院,浙江舟山316004 [2]浙江海洋学院海洋科学学院,浙江舟山316004

出　　处：《浙江海洋学院学报（自然科学版）》2008年第4期361-365,共5页Journal of Zhejiang Ocean University(Natural Science Edition)

基　　金：浙江省重大科技专项农业项目(2008C12029);浙江省自然科学基金资助项目(Y305119)

摘　　要：采用曲霉CJ22-326所产壳聚糖酶降解壳聚糖,得到酶解产物-壳寡糖。采用薄层层析法(TLC)对壳寡糖进行分离。结果显示,薄层层析的最佳条件是:溶剂系统,正丁醇/水/乙酸/氨水,体积比为10:5:5:1;温度为25℃。用0.5%茚三酮溶液显色,其效果最佳。在此条件下利用硅胶制备板,可以分离和制备单体的壳寡糖。Through thin layer chromatography （TLC） to separate the chitosan enzyme Aspergillus CJ22-326 degradation product,the purified chitooligosaccharides was obtained. The results showed that Butanol, water, acid and ammonia is the optimal solvent system, the best condition is butanol, water, acetic acid, ammonia volume ratio of 10：5：5：1, temperature of 25 ℃. Using the 0.5% Ninhydrin solution to color, the effect is best. Under these conditions, the use of silica gel plates preparation, and preparation can be separated from the monomer chitooligosaccharides.

关 键 词：壳聚糖 壳寡糖 薄层层析 分离 分析 

分 类 号：O652.63[理学—分析化学]