壳聚糖固定化血管紧张素转化酶及其性质  

Immobilization of Angiotensin Converting-enzyme on Chitosan Microspheres

作　　者：洪韫嘉[1] 李谭瑶[1] 陈波[1] 姚守拙[1] 

机构地区：[1]湖南师范大学化学生物学及中药分析省部共建教育部重点实验室,长沙410081

出　　处：《高等学校化学学报》2009年第2期328-331,共4页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划(批准号:2006CB504701);国家自然科学基金(批准号:20875028)资助

摘　　要：以壳聚糖微球为载体,戊二醛为交联剂固定化血管紧张素转化酶,研究了酶固定化的最优条件和固定化酶的性质.结果表明,在戊二醛质量分数为2.5%、给酶量为8mg/mL时,固定化酶的比活性最大,为0.085U/g.固定化酶在40～50℃,pH在7～9之间有最大活性,其米氏常数Km为2.39mmol/L.同时,固定化酶具有良好的稳定性,可重复利用.Chitosan is a polysaccharide which is widely used in enzyme immobilization as a support. Angiotensin converting-enzyme（ACE） is an important member of the rennin-angiotensin-aldosteronesystem which could cause high blood pressure. In this study, ACE was immobilized on chitosan microspheres with glutaraldehyde as cross-linking reagent. The optimum conditions and properties of immobilization enzyme were investigated. The max ratio activity was 0. 085 U/g under 2.5% glutaraldehyde and 8 mg/mL soluble enzyme for immobilized enzyme. The optimum pH, temperature and K,n of the immobilized enzyme were 7---9, 40--50℃ and 2.39 mmol/L. The immobilized ACE show higher thermal stability and can be used repeatedly to a certain extent. It was a fundamental study in order to develop the way of screening ACE inhibitor.

关 键 词：壳聚糖 血管紧张素转化酶 固定化酶 

分 类 号：O629.8[理学—有机化学] O657.7[理学—化学]