纸尖法和刮匙法对龈下牙周致病菌检出比较研究  

Comparison of different sampling techniques for detection of periodontopathogenic bacteria in subgingival plaque samples

作　　者：王丽[1] 杨禾[2] 吴亚菲[2] 

机构地区：[1]徐州医学院口腔医学系,江苏徐州221004 [2]四川大学华西口腔医学院牙周科

出　　处：《临床口腔医学杂志》2008年第12期707-710,共4页Journal of Clinical Stomatology

基　　金："十五"国家科技攻关计划项目(No2004BA720A26)

摘　　要：目的:比较纸尖法和刮匙法对慢性牙周炎患者龈下菌斑中牙龈卟啉单胞菌、福赛斯坦纳菌、伴放线菌嗜血菌、具核梭杆菌检出的影响。方法:对30例慢性牙周炎患者分别用纸尖和刮匙法收集4个区域牙周袋探诊最深位点龈下菌斑,采用16SrRNAPCR方法检测两种方法收集到的龈下菌斑样本中的牙龈卟啉单胞菌、福赛斯坦纳菌、伴放线菌嗜血菌、具核梭杆菌。结果:两种方法一致性较好,纸尖法与刮匙法无显著差异(P>0.05)。结论:纸尖法与刮匙法均为比较准确的取样方法。Objective： The aim of this study was to compare the paper point and curette for detection of periodontopathogenic bacteria in subgingival plaque samples. Method： Subgingival plaque were taken by paper point and curette from the deepest pockets in each sextant in 30 patients,Porphyromonas gingivalis （P.gingivalis）,Tannerellaforsythia （T. forsythensis ） ,Haemophilus actinomycetemcomitans （H.actinomycetemcomitans ） and Fusobacterium nucleatum （F. nucleaturn） were identified by 16S r RNA polymerase chain reaction. Result：There were no significant qualitative differences between paper point and curette for detection of P.gingivalis,F. nucleatum and T. forsythensis. Conclusion：Both techniques seem to be suitable for microbiological diagnostics.

关 键 词：纸尖法 刮匙法 牙周致病菌 

分 类 号：R781.4[医药卫生—口腔医学]