新生牛牛脑活性肽NBBP-1对人神经母细胞瘤SK-N-SH细胞增殖和相关基因表达的影响  

The Effects of Neonatal Bovine Brain Bioactive Peptide NBBP-1 on Proliferation and Associated Gene Expression of Human Neuroblastoma SK-N-SH Cells

作　　者：刘用金[1] 李祺福[1] 黄晓明[1] 石松林[1] 宋建晔[1] 梁盈[1] 武福云[1] 

机构地区：[1]厦门大学生命科学院细胞生物学研究室细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《现代生物医学进展》2009年第2期208-211,222,共5页Progress in Modern Biomedicine

基　　金：福建省科技计划重点项目(2003N0052)资助

摘　　要：目的:应用新生牛牛脑活性肽NBBP-1处理人神经母细胞瘤SK-N-SH细胞,观察NBBP-1对人神经母细胞瘤SK-N-SH细胞增殖和相关基因表达的影响。方法:通过细胞计数、流式细胞仪、光学显微镜、免疫细胞化学方法检测细胞的变化。结果:经60μg/mLNBBP-1处理后,SK-N-SH细胞生长抑制率高达90.09%,细胞周期被阻滞在G0/G1,细胞核裂解成多个,免疫细胞化学染色结果显示,经处理后bcl-2抗凋亡基因蛋白表达减弱,而p53、fas、bax等促凋亡基因蛋白表达增强。结论:新生牛牛脑活性肽NBBP-1对人神经母细胞瘤SK-N-SH细胞凋亡具有显著的诱导作用,其诱导癌细胞凋亡的机理与其调节和干预癌基因bcl-2和p53、fas、bax等抑癌基因的表达有关。Objective： To study the effects of neonatal bovine bioactive brain peptides in peak I （NBBP-1）, with which we treated SK-N-SH cells, on the proliferation and gene expression of human neuroblastoma SK-N-SH cells. Methods： The changes of SK-N-SH cells induced by NBBP-1 were detected by means of cell count, flow cytometry analysis, light microscope and immunocytochemistry. Results： Treated with 60μg/mL NBBP-1, the proliferation of SK-N-SH cells was inhibited effectively, and the rate was up to 90.09 %. Cell cycle was arrested in G1/S phase. Nucleus broke into several parts. The results of immunocytochemistry showed that expression of anti-apoptotic gene bcl-2 was down-regulated and expression of apoptotic genes p53, fas, bax were up-regulated. Conclusions： This study suggested that NBBP-1 could regulate the proliferation of human neuroblastoma SK-N-SH cells effectively, and the effect was related with the changes of oncogene and tumor-suppressor gene.

关 键 词：新生牛牛脑活性肽 SK-N-SH细胞 细胞凋亡 

分 类 号：Q279[生物学—细胞生物学] R730.264[医药卫生—肿瘤]