金地鼠颊囊致癌模型P_(53)及其对细胞增殖作用的观察  

Observations of P_(53) and the role of P_(53) in the cell proliferation on golden hamster carcinogenesis model

作　　者：钟文静[1] 周曾同[1] 曹宏康[1] 汪铮[1] 乐福媛[1] 周永梅[1] 

机构地区：[1]上海第二医科大学口腔医学院,上海200011

出　　处：《临床口腔医学杂志》1998年第1期32-34,共3页Journal of Clinical Stomatology

摘　　要：有效的早期诊断是阻断癌前病变、预防癌变发生的必要条件。本实验建立DMBA(二甲基苯并蒽)诱导的金地鼠颊囊致癌模型,采用P_(53)、增殖细胞核抗原(PCNA)等免疫组化方法及VIDAS图像分析,发现P_(53)表达能敏感、准确地反映口腔癌前病变的粘膜病变程度,并观察到P_(53)阳性细胞在不同程度的上皮异常增生间具有不同的分布形态;实验还发现P_(53)与PCNA从不同检测角度揭示了细胞动力学变化的同一本质,初步证实了“P_(53)功能失活后促进了细胞的异常增殖”这一设想;实验结果还提示控制固定时间、注意观察与计数等因素在实验中的重要性。The effective early diagnosis is essential to obstructing oral precancerous lesions and preventing carcinomatous conversion. An immunohistochemistry with anti-P53 antibody and anti-PCNA antibody. VIDAS image analysis system were applied and observed on golden hamster cheek pouch car-cinogenesis model. The results showed that expression of P53 protein could reflect the severity degrees of the lesions sensitively and accurately, moreover, the distribution of P53-positive cells varied with the grade of epithelial dysplasia: P53 and PCNA disclosed the same essence of cellular dynamics from different points of view, and verified the hypothesis preliminarily, 'Once being in loss of function. P53 can promote abnormal cell growth. '; the results also indicated that it was important to control the time of fixation and select the correct way of observation and counting.

关 键 词：金地鼠颊囊 致癌模型 P53 细胞增殖 动物模型 

分 类 号：R739.85[医药卫生—肿瘤]