血管细胞黏附分子-1在口腔鳞状细胞癌中的表达及其意义  

Expression and significance of vascular cell adhesion molecule-1 in human oral squamous cell carcinoma

作　　者：孙乐刚[1] 王芳[1] 刘玲[2] 宋宇峰[3] 杨佑成[1] 王丽芳[1] 

机构地区：[1]滨州医学院附属医院口腔外科 [2]滨州医学院附属医院胃镜室,山东滨州256603 [3]贵阳医学院附属医院口腔外科,贵州贵阳550004

出　　处：《华西口腔医学杂志》2008年第6期591-594,共4页West China Journal of Stomatology

基　　金：贵州省第二批跨世纪人才省政府专项基金资助项目(黔科合人专字2000-9813号);贵州省优秀科技教育人才省长专项基金资助项目[黔科教办(2004)07号]

摘　　要：目的研究血管细胞黏附分子-1(VCAM-1)在口腔鳞状细胞癌(OSCC)中的表达与定位及其临床意义。方法应用分子原位杂交和免疫组化方法对48例OSCC组织和10例正常口腔黏膜组织中VCAM-1mRNA和VCAM-1蛋白质的表达和定位进行检测,比较VCAM-1在不同口腔组织中的表达率及其与临床指标的关系。结果VCAM-1蛋白定位于肿瘤细胞胞浆和胞膜,VCAM-1mRNA定位于肿瘤细胞胞浆。VCAM-1mRNA和VCAM-1蛋白在OSCC中的表达率均显著高于正常口腔黏膜组织(P<0.01),VCAM-1mRNA表达与VCAM-1蛋白表达呈正相关(P<0.01);OSCC中淋巴结转移者VCAM-1蛋白的表达显著高于无淋巴结转移者(P<0.01)。结论OSCC中VCAM-1基因的高表达可能与肿瘤的浸润和转移有关。Objective To study the expression and the location of vascular cell adhesion molecule-1 （VCAM-1） gene and its clinical significance in human oral squamous cell carcinoma （OSCC）. Methods In situ hybridization, PV-9000 polymer detection system for immunohistochemical staining was used to detect the expression and the location of VCAM-1 mRNA and VCAM-1 protein in 48 cases of OSCC and 10 cases of normal controls. Statistical analysis was performed using chi-square test in SPSS 13.0. Results VCAM-1 protein was mainly expressed in tumor cell cytoplasm and membrane, VCAM-1 mRNA was mainly expressed in tumor cell cytoplasm. The expression rate of VCAM-1 mRNA and VCAM-1 protein was significantly higher in OSCC than that in normal oral mucosa（P〈0.01）. The expression of VCAM-1 mRNA was positively correlated with that of VCAM-1 protein （P〈0.01）. In the clinicopathologic factors, lymph node metastasis and depth of infiltration were closely correlated with VCAM-1 expression （P〈0.01）. The expression of VCAM-1 was significantly higher in tumor with lymph node metastasis than in tumor without lymph node metastasis （P〈0.01）. Conclusion Overexpression of VCAM-1 gene in OSCC may play a potential role in the development of OSCC. The overexpression of VCAM-1 gene in OSCC may be related to the tumor infiltration and metastasis.

关 键 词：口腔鳞状细胞癌 血管细胞黏附分子-1 原位杂交 免疫组化染色 

分 类 号：R739.8[医药卫生—肿瘤]