一株芽孢杆菌及其破乳特性研究  

Demulsification of Bacillus sp.

作　　者：王晓梅[1] 李大平[1] 彭世群[1] 何晓红[1] 田崇民[1] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041

出　　处：《环境科学与技术》2009年第3期22-26,共5页Environmental Science & Technology

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-SW-114);中国石油化工股份有限公司专题

摘　　要：从某油污样品中分离、筛选出一株破乳菌株10^#，经鉴定属芽孢杆菌属。该菌株对油田稳定O／W乳化液具明显的破乳效果。该菌株最佳培养条件为：温度30-35℃，pH7．0-7．5，生长时间48-72h，碳源为液体石蜡。该菌株在培养48h后具有明显而稳定的破乳效果，破乳活性部位为菌体。A bacterium strain named 10^# was isolated and screened from oil-contaminated samples, which was identified as Bacillus sp. Strain 10^# exhibited a high degree of demulsification activity against the oil-in-water（o/w） emulsion of petroleum industry. The optimum growth conditions of the strain 10^# were temperature 30-35℃, pH 7.0-7.5, time 48-72h, with atoleine as carbon source. Strain 10^# had powerful and stable demulsification activity after growing for 48h. Only thalli had demulsification activity.

关 键 词：生物破乳 O/W乳化液 芽孢杆菌 

分 类 号：X172[环境科学与工程—环境科学]