HPLC测定拉米夫定中的右旋异构体  

Determination of dextroisomer in Lamivudine by HPLC

作　　者：吴意红[1] 张丹[1] 毛惠英[1] 唐丽红[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2009年第1期80-81,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用HPLC法测定拉米夫定中的右旋体。方法采用CyelobondI2000色谱柱（250mm×4．6mm，5μm），甲醇-0．1mol-L-醋酸铵（5：95）为流动相，流速0．8ml·min-1，柱温10℃～30℃，检测波长270nm。结果拉米夫定的光学异构体得到了基线分离，其右旋体0．5～5μg·ml。与峰面积的线性关系良好（r=0.9995），最低检测限为1．5ng。结论所建方法准确、简便、快速，适合于拉米夫定中右旋体的测定。OBJECTIVE To develop an HPLC method for determination of dextroisomer in Lamivudine. METHODS A cyclobond I 2000 column （250 mmx 4. 6 mm ,5 μm） was used with the mobile phase of methanol -0. 1 mol· L- 1 ammonium acetate （5 ： 95 ） at a flow rate of 0. 8 ml-min-1 and the temperature of the column was 10℃ -30℃ with a detective wavelength at 270 nm. RESULTS The separation of both enantiomers of Lamivudine could be achieved baseline resolution. The calibration curve for Lamivudine dextroi- somer exhibited an excellent linear relationship with a correlation coefficient of 0. 9995 in concentration range of 0. 5 - 5.0 μg· ml -1 The detection limit was 1.5 ng for lamivudine dextroisomer. CONCLUSION The method is accurate, simple, quick and suitable for determination of Lamivudine dextroisomer.

关 键 词：拉米夫定 光学异构体 右旋体 高效液相色谱法 Β-环糊精 

分 类 号：R917[医药卫生—药物分析学]