内皮素1对人A375黑素瘤细胞株细胞黏附及细胞黏附分子1表达的影响  

Effects of endothelin-1 on the adhesion and expression of intercellular adhesion molecule 1 by A375 human malignant melanoma cells

作　　者：刘志军[1] 张焕梅 段明[1] 

机构地区：[1]南华大学附属第一医院皮肤科,湖南衡阳421001 [2]广东省江门市皮肤病防治所

出　　处：《中华皮肤科杂志》2009年第2期105-107,共3页Chinese Journal of Dermatology

基　　金：基金项目：湖南省医药卫生科研计划项目（B2007099）;衡阳市科技发展项目（2007KJ017）

摘　　要：目的探讨内皮素（ET）-1对人A375黑素瘤细胞增殖、黏附、迁移及细胞黏附分子（ICAM）-l表达的影响。方法MTT法观察ET-1对人A375黑素瘤细胞生长的影响，黏附实验检测细胞黏附，Transwell迁移系统检测细胞迁移，流式细胞仪检测对人A375黑素瘤细胞ICAM-1表达的影响。结果ET-1在0．002～0．2μg／mL浓度范围可促进黑素瘤细胞的增殖、在纤连蛋白上的黏附、通过微孔滤膜及抑制ICAM—1的表达（P〈0．01），在0．2μg／mL时作用最强，细胞代谢活性（24h）、细胞黏附率、ICAM-1含量（24h）分别为0．327±0．009、163．31±4．05、4．667±0．551；在0．2～2μg／mL浓度范围作用相反。结论ET-1可能通过抑制ICAM—1的表达、促进细胞增殖、黏附及迁移而增强细胞代谢、增加色素形成。Objective To observe the effect of endothelin-1 （ET-1） on the cell growth, adhesion, migration and expression of intercellular adhesion molecule 1 （ICAM-1） by A375 human malignant melanoma cells. Methods A375 cells were cultured in the presence of ET-1 of various concentrations （0.002, 0.02, 0.2, 2 μg/mL） for different periods. MTT method and flow cytometry were applied to detect the proliferation and ICAM-1 expression of these cells, respectively, after 24-, 48-, and 72-hour treatment. After 24-hour treatment, the cell adhesion and migration of A375 cells were assessed with cell adhesion assay and Transwell chambers, respectively. Results In the case of ET-1 from 0.002 to 0.2 μg/mL, it enhanced the proliferation, adhesion, migration of A375 cells and inhibited the expression of ICAM-1 by A375 cells in a dose dependent manner （P 〈 0.01 or 〈 0.05）; however, for ET-1 of 2 μg/mL, the situation was the opposite. Moreover, after 24-hour culture with ET-1 of 0.2 μg/mL, the metabolic activity, cell adhesion rate, and expression of ICAM-1 peaked at 0.327 ± 0.009, （163.31±4.05）% and 4.667 ±0.551, respectively. Conclusion ET-1 may enhance cellular metabolism and pigmentation by suppressing the expression of ICAM-1 and promoting the proliferation, adhesion and migration of melanoma cells.

关 键 词：黑色素瘤 实验性 内皮缩血管肽I 细胞黏附分子 

分 类 号：R739.5[医药卫生—肿瘤]