基于线粒体COI基因序列探讨泥蚶的遗传分化  

Genetic Differentiation of Tegillarca granosa Based on Mitochondrial COI Gene Sequences

作　　者：郑文娟[1] 朱世华[1,2] 沈锡权[2] 刘必谦[1] 潘志崇[2] 叶央芳[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211 [2]宁波大学生命科学与生物工程学院生物系,浙江宁波315211

出　　处：《Zoological Research》2009年第1期17-23,共7页动物学研究（英文）

基　　金：浙江省教育厅资助项目(20000008)

摘　　要：采用PCR技术对我国沿海地区7个泥蚶群体的线粒体COI基因部分序列进行了测定和遗传分析。在来自7个群体的38个泥蚶样本均得到660bp的COI基因片段序列,共103个多态位点,组成17种单倍型;数据分析表明:7个群体形成了二大类群:福建以北(包括福建)的5个群体(江苏盐城、浙江奉化、浙江乐清养殖和自然群体、福建福鼎)形成一个类群,类群内的遗传距离为0.0016;福建以南的类群(广东湛江、海南海口)形成一个类群,遗传距离为0.0006;二个类群之间的遗传距离为0.1529,表现为高度的分化。因此我国沿海泥蚶已分化形成福建以南和以北二大类群,二大类群之间的遗传分化已达到亚种水平。Partial sequences of the mitochondrial DNA cytochrome c oxidase subunit Ⅰ gene （COI） of seven Tegillarca granosa populations, which were collected from China＇s coastal areas, were amplified by polymerase chain reaction （PCR）. The length of COI gene of 38 Tegillarca granosa individuals from seven populations was all 660bp. One hundred and three variable sites were detected in the nucleotide sequences of 660 bp, and 17 different haplotypes were identified. The result showed that the seven populations could be divided into two groups based on the the genetic distance and phylogenetic analysis of their COI gene sequences, The two groups were classified as Group in the Northern Fujian （including Fujian） and Group in the Southern Fujian, Group in the Northern Fujian was composed of five populations and the genetic distance was 0.0016. Group in the Southern Fujian was composed of two populations and the genetic distance was 0.0006. However, the genetic distance between the two groups was significantly high （0.1529）, which suggested significant genetic differentiation between the two groups. It suggested that Group in the North of Fujian （including Fujian） and Group in the South of Fujian should be the same species, but they were different subspecies.

关 键 词：泥蚶 COI基因 遗传多样性 遗传分化 

分 类 号：S917.4[农业科学—水产科学]