超重人群网膜脂肪组织脂联素mRNA表达水平的性别差异  

Gender Differences in Adiponectin mRNA Expression in Omental Adipose Tissue of Subjects with Obesity

作　　者：班东杰[1] 杜秉权[1] 赵紫琴[1] 雒蓉[1] 安玲[1] 

机构地区：[1]天津市公安医院,300042

出　　处：《天津医药》2009年第2期84-87,共4页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(项目编号:07JCYBJC11000)

摘　　要：目的:探讨超重人群网膜(OM)脂肪组织脂联素(APN)mRNA表达水平的性别差异。方法:选取女性超重患者26例(BMI≥25kg/m2,OBF组)、男性超重患者22例(BMI≥27kg/m2,OBM组)以及正常体质量者40例(BMI<25kg/m2,女性为NF组,男性为NM组),用TaqMan探针实时荧光定量PCR检测腹部OM脂肪组织中APNmRNA的表达,并测定血糖、血清胆固醇(TC)、血清三酰甘油(TG)、血清胰岛素等指标,计算胰岛素抵抗指数(HOMA-IR)。结果:(1)NF组OM脂肪组织APN基因表达量(OAmR)较NM组有升高的趋势,但差异无统计学意义(P>0.05);OBF组OAmR明显高于OBM组(P<0.05),但OBF组APN基因表达量仍低于NF组(P<0.01);(2)OAmR与BMI、腰臀比(WHR)和TC水平负相关(r分别为-0.262、-0.143、-0.149,均P<0.05);超重人群中,OAmR与性别呈正相关(r=0.259,P<0.05)。在OBF组中,OAmR与BMI、LDL-C呈负相关(r分别为-0.320、-0.339,P<0.05)。结论:女性超重人群OAmR高于超重男性,性别与脂肪组织APN的表达量之间的联系更多的表现在超重人群,体质量适中者则无此关联。Objective： To explore the gender differences in adiponectin mRNA expression in omental （OAmR） adipose tissue of subjects with obesity. Methods： Twenty-six obese women with OM adipose tissue （BMI ≥25 kg/m^2, OBF group）, 22 obese man with OM adipose tissue （BMI≥27 kg/m^2, OBM group）, and 40 subjects with normal weight （BMI 〈 25 kg/m^2, NF and NM group） were involved in this study. The levels of OAmR were compared by real-time quantitative RT-PCR with TaqMan probe. Serum glucose, cholesterol, triglyceride （TG） and insulin resistance index by HOMA （HOMA-IR）were determined. Results： （1） OAmR in NF group was higher than that in NM group, but there was no statistical significance （P 〉 0.05）. OAmR in OBF group was significantly higher than that in OBM group （P 〈 0.05）. OAmR in OBF group was significantly lower than that in NF group （P 〈 0.01）. （2） OAmR was negatively correlated with BMI, WHR, and TC （r= -0.262, r = -0.143, r = -0.149, P 〈 0.05）, and was positively correlated with gender in obesity （r = 0.263 ,P 〈 0.05 ）. There was a significantly negative correlation between OAmR and BMI ,LDL-C in OBF group （r =-0.320, r =-0.339, P 〈 0.05）. Conclusion： OAmR in obese women is higher than that in obese men. OAmR is more closely related to gender in obese subjects, and there is no association in lean subjects.

关 键 词：脂联素 性别因素 脂肪组织 网膜 基因表达 肥胖症 

分 类 号：R734.2[医药卫生—肿瘤] R181.13[医药卫生—临床医学]