维生素K3诱导人肝癌细胞SMMC-7721损伤中Bcl-2和Bax表达的变化及意义  

Changes of Bcl-2 and Bax on vitamin K3 induced injury in human hepatocarcinoma SMMC-7721 cells

作　　者：杨闯[1] 张宏宇[2] 孔晓霞[2] 孙连坤[2] 郑宇[3] 王广义[1] 

机构地区：[1]吉林大学第一医院普通外科,吉林长春130021 [2]吉林大学基础医学院病理生理学教研室,吉林长春130021 [3]黑龙江省哈尔滨市第一医院普通外科,黑龙江哈尔滨150010

出　　处：《吉林大学学报（医学版）》2009年第1期108-111,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200507083);吉林省政府科研基金资助课题(20071500)

摘　　要：目的:探讨Bcl-2、Bax在维生素K3(VK3)诱导人肝癌细胞SMMC-7721损伤过程中的变化,为研究VK3诱导肝癌细胞损伤的机制提供参考。方法:体外培养SMMC-7721细胞,取对数生长期细胞分为对照组和不同浓度VK3处理的实验组(20、40和60μmol.L-1),MTT法检测各组SMMC-7721细胞生存率;紫外分光光度法检测乳酸脱氢酶(LDH)释放率;RT-PCR检测Bcl-2及Bax mRNA表达水平;Western blotting检测Bcl-2及Bax蛋白表达水平。结果:与对照组比较,20μmol.L-1VK3组细胞存活率、LDH释放率、Bcl-2和Bax mRNA表达量及其蛋白表达量未见明显变化;与对照组比较,40和60μmol.L-1VK3组细胞存活率降低(P<0.05或P<0.01);LDH释放率增加(P<0.05或P<0.01);Bcl-2 mRNA表达率降低(P<0.05);BaxmRNA表达量率升高(P<0.05);Bcl-2蛋白表达水平下降(P<0.05);Bax蛋白表达水平升高(P<0.05)。结论:40μmol.L-1剂量的VK3能够诱导SMMC-7721细胞损伤,其机制可能与下调Bcl-2和上调Bax蛋白表达有关。Objective To investigate the changes of Bcl-2 and Bax expressions on human hepatocarcinoma SMMC- 7721 cells injuried with vitamin K3 （VK3） treatment and provide information for further research of its mechanism. Methods SMMC-7721 cells were cultivated in vitro, then were divided into control group and experimental groups treated with different concentrations of VK3 （20, 40 and 60 μmol · L^-1 ）. The viability of SMMC-7721 cells treated with VK3 was measured by MTT assay, LDH release rate was detected by ultraviolet spectrophotometry, the expressions of Bcl-2 and Bax mRNA were determined using RT-PCR, the expressions of Bcl-2 and Bax protein were assayed by using Western Blotting. Results Compared with control group, 20 μmol· L^-1 VK3 did not induce significant changes of cell viability, LDH release rate, the expressions of Bcl-2 and Bax mRNA, the expressions of Bcl-2 and Bax protein. In 40 and 60μmol · L^-1 VK3 groups the cell viabilities decreased （P〈0.05 or P〈0. 01）, the LDH release rates increased （P〈0. 05 or P〈0. 01）, the expressions of Bcl-2 mRNA decresed （P〈0. 05）, the expressions of Bax mRNA increased （P〈0. 05）, the expressions of Bel-2 protein decresed, the expression of Bax protein increased compared with control group. Conclusion 40 μmol · L^-1 VK3 can induce SMMC-7721 cell injury, its mechanism may be related to down-regulation of Bcl-2 protein expression and upregulation of Bax protein expression.

关 键 词：维生素K3 Bcl-2 BAX SMMC-7721细胞 细胞损伤 

分 类 号：R735.7[医药卫生—肿瘤]