杜氏盐藻核基质结合区结合蛋白的细胞定位  

Cellular localization of MAR binding protein of Dunaliella salina

作　　者：王鹏举[1] 王天云[2] 冯英才[1] 刘红涛[1] 薛乐勋[1] 

机构地区：[1]郑州大学细胞生物学研究室,郑州450052 [2]新乡医学院生物化学与分子生物学教研室,河南新乡453003

出　　处：《广西植物》2009年第1期55-58,共4页Guihaia

基　　金：科技部国际科技合作项目(2007DFA01240);国家自然科学基金(30470030)~~

摘　　要：为研究核基质结合区结合蛋白的功能及调控机制,PCR扩增杜氏盐藻MBP的cDNA全长序列及N端和C端序列,与绿色荧光蛋白基因融合构建真核表达载体,脂质体转染CHO细胞,Western blotting和荧光显微镜检测基因表达情况和细胞定位。结果显示:MBP及N端和C端融合蛋白成功在CHO细胞表达,MBP和C端部分定位于细胞核且聚集于核仁,N端部分分布整个细胞,说明MBP定位于细胞核且细胞定位信号位于C端,MBP可能与rRNA前体结合发挥作用。To study the function of Matrix attachment region（MAR）binding protein（MBP）, full length, N-terminal and C-terminal of MBP gene from Dunaliella salina were amplified by RT-PCR,and fused to the green fluorescence protein gene to construct the eukaryotic vector. Then,the recombinant vectors were transfected into Chinese hamster ovary（CHO） cells by Lipofectamine 2000. Fluorescence microscope and western blotting were performed to determine the expression of the fusion proteins and cellular localization. The results showed that the fusion proteins were expressed in CHO cells, the proteins of MBP and C-terminal part were localized in the nucleus and clearly detected in nucleoli,however, the N-terminal part was distributed into all ceils.

关 键 词：杜氏盐藻 核基质附着区 核基质结合区结合蛋白 真核表达载体 细胞定位 

分 类 号：Q781[生物学—分子生物学]