非小细胞肺癌细胞系中DAL-1表达与其启动子甲基化的关系  

Relationship between DAL-1 expression and promoter methylation of DAL-1 gene in non-small cell lung carcinoma

作　　者：徐若冰[1] 张雅洁[2] 郭爱林[3] 

机构地区：[1]南方医科大学南方医院病理科,广东广州510515 [2]广州医学院病理学教研室,广东广州510182 [3]广东省人民医院医学研究中心生物芯片部,广东广州510080

出　　处：《中国现代医学杂志》2009年第2期185-188,共4页China Journal of Modern Medicine

基　　金：广东省科技计划项目(No:2004B30601011);广东省自然科学基金(No:7003068);广州市科技计划项目(No:61002)

摘　　要：目的探讨非小细胞肺癌中潜在抑癌基因DAL-1的表达与其启动子甲基化的关系。方法10例非小细胞肺癌初诊患者,每例在治疗前抽取胸水并分离培养肿瘤细胞成系。分别采用逆转录-聚合酶链反应(RT-PCR)、western blot检测DAL-1的mRNA水平和蛋白水平的表达,用甲基化特异性PCR检测DAL-1基因启动子的甲基化。结果10个细胞系中有9个完全不表达DAL-1mRNA和蛋白,仅有1个表达与人支气管上皮细胞株HBE水平相当。9个不表达DAL-1的细胞系有6个检出启动子CpG岛甲基化。结论DAL-1在大多数非小细胞肺癌患者胸水分离细胞系中表达缺失,而DAL-1启动子甲基化是造成这种现象的主要原因。[Objective] To investigate the possible mechanism of carcinogenesis and invasion of non-small cell lung carcinoma （NSCLC）. [Methods] Pleural fluid was collected from cases of primary NSCLC. Tumor cells were separated from pleural fluid and euhured to be stable cell lines. There are 10 cell lines successfully etdtured. Expression of DAL-1 （Differentially expressed in Adenocareinoma of the Lung） mRNA was detected by RT-PCR.Expression of DAL-1protein was detected using Western blot. Methylation of DAL-1 gene promoter was detected using methylation-speeifie PCR （MSP）. [Results] Loss of DAL-1 Promoter protein expression was found in 9/10 cell lines. Methylation of DAL-1 could be detected in 6 cell lines which have no DAL-1 expression. [Conclusions] Loss of expressions of DAL-1 can be detected in most invasive cell lines which separated from pleural fluid. Methylation of the promoter is an important reason of the loss and low expression of DAL-1 gene and protein.

关 键 词：非小细胞肺癌 DAL-1 甲基化 

分 类 号：R734.2[医药卫生—肿瘤]