滋阴清热中药对生长延滞中骺板软骨细胞血管内皮生长因子表达的影响  

Effects of Nourishing yin clearing heat (滋阴清热) Chinese herbal medicine on vascular endothelial growth factor in the epiphyseal cartilage of growth retardation

作　　者：沈欢嗣[1] 陈朝蔚[1] 李玉梅[1] 陈永强[1] 

机构地区：[1]上海市中医医院骨伤科,上海200071

出　　处：《中国骨伤》2009年第1期14-17,共4页China Journal of Orthopaedics and Traumatology

基　　金：上海市教委重点学科建设项目资肋(编号:05cz34)

摘　　要：目的:通过检测滋阴清热中药对地塞米松诱导的生长延滞中骺板软骨细胞血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,探讨滋阴清热中药拮抗地塞米松诱导的生长延滞机制。方法:1月龄新西兰兔30只,随机分为3组:正常对照组、地塞米松组和中药治疗组,每组10只。地塞米松组和中药治疗组给予地塞米松5mg/kg,每天肌肉给药。中药治疗组给予混有滋阴清热中药的颗粒饲料,正常组及地塞米松组均给予普通饲料,分别在给药第6、12周时处死。TUNEL染色法检测细胞凋亡指数。免疫组织化学染色测定生长板软骨细胞中VEGF表达的阳性指数,实时荧光定量PCR检测VEGF mRNA在各组的表达。结果:6、12周地塞米松组软骨细胞凋亡指数大于正常组,差异有统计学意义(P<0.01)。6、12周中药治疗组软骨细胞凋亡指数小于地塞米松组,差异有统计学意义(P<0.01)。6、12周地塞米松组VEGF的阳性指数、VEGF mRNA表达水平明显低于正常组,差异有统计学意义(P<0.01),中药治疗组VEGF阳性指数、VEGF mRNA表达水平明显高于地塞米松组,差异有统计学意义(P<0.01)。结论:滋阴清热中药可减少因长期大剂量使用糖皮质激素导致的软骨细胞凋亡;滋阴清热中药可能是通过调控生长板软骨细胞中VEGF而减轻地塞米松对生长板的抑制作用。Objective：To observe the effects of Nourishing yin clearing heat （滋阴清热） Chinese herbal medicine on vascular endothelial growth factor （VEGF） in growth retardation induced by Decamethasone and observe its mechanisms. Methods ：Thirty one-month-old New Zealand white rabbits were randomly divided into normal group, dexamethasone-treated group and Nourishing yin clearing heat （滋阴清热） Chinese herbal medicine-treated group. The rabbits in dexamethasone group and Nourishingyin clearing heat （滋阴清热） Chinese herbal medicine-treated group received dexamethasone （5 mg/kg·d）. The rabbits were sacrificed at the 6th and 12th week after administration, and then rabbit tibia articular was removed. ①Using TUNEL stain to detect apoptotic index. ②Using immunohistochemical stain to detect the positive index of the expression of vascular endothelial growth factor（VEGF） in the epiphyseal cartilage of growth. ③Using fluorescent quantitative PCR to detect the expression intensity of VEGF mRNA in each group. Results：At the 6th and 12th week after administration, there were significant difference in apoptotic index and cell proliferation index between dexamethasone group and normal group （P〈0.01, dexamethasone group more than normal group）. Immunohistochemical stain and fluorescent quantitative PCR indicated that the expression of VEGF and VEGF mRNA in dexamethasone group was significantly decreased as compared with that in normal group （P〈0.01）, and also obviously lower than Chinese herbal medicine-treated group （P〈0.01）. Conclusion：VEGF has an important role during the growth retardation induced by Dexamethasone. Nourishing yin clearing heat （滋阴清热） Chinese herbal medicine can reduce the growth retardation induced by Dexamethasone through increasing the VEGF expression in growth plate chondrocytes and then increase angiogenesis.

关 键 词：滋阴清热 中草药 地塞米松 生长面 骺软骨 血管内皮生长因子类 

分 类 号：R735.2[医药卫生—肿瘤] R684.302[医药卫生—临床医学]