结核分枝杆菌杂交信号放大检测方法的建立  

Development of Hybridization Signal Amplification Method for Detection of Mycobacterium tuberculosis

作　　者：王海河[1] 赵春燕[1] 李凡[1] 

机构地区：[1]吉林大学白求恩医学院病原生物学教研室,长春130021

出　　处：《中国生物制品学杂志》2009年第2期185-187,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助课题(30672007)

摘　　要：目的建立结核分枝杆菌杂交信号放大检测方法。方法构建纳米颗粒信号放大载体,建立杂交信号放大方法,检测标本中结核分枝杆菌特异性的插入序列IS6110。应用该方法检测124份临床结核患者标本,并与细菌培养和生化鉴定法的检测结果进行比较,确定该方法的灵敏度和特异性。结果杂交信号放大方法检测临床标本的灵敏度为87.7%,特异性为92.2%,假阳性率为7.8%,假阴性率为12.3%。结论已建立具有较高灵敏度和特异性的杂交信号放大检测方法,该方法操作简便、快速,可作为结核分枝杆菌临床标本的检测方法。Objective To develop a hybridization signal amplification method for detection of Mycobacterium tuberculosis. Methods Nanoparticle signal amplification carrier was prepared, based on which a signal amplification method was developed to detect the specific inserted sequence IS6110 in M. tuberculosis. A total of 124 specimens from patients with tuberculosis were detected, and the results were compared with those by bacterial culture and biochemical assay to evaluate the sensitivity and specificity of the developed method. Results The sensitivity, specificity, false positive rate and false negative rate of the developed method for detection of clinical specimens were 87.7%, 92.2%, 7.8% and 12.3% respectively. Conclusion A rapid, sensitive and specific hybridization amplification method for detection of M. tuberculosis was developed, which was simple and easy to handle, and suitable for clinical specimens.

关 键 词：杂交信号 放大 结核分枝杆菌 

分 类 号：R378.911[医药卫生—病原生物学] Q93-332[医药卫生—基础医学]