HO-1参与葛根素对抗高糖诱导的大鼠血管舒张功能下降  

Puerarin protects high glucose-induced injury of vascular relaxation by activation of heme oxygenase-1

作　　者：倪超[1] 孟香红[1] 范莹[2] 朱一苗[1] 朱立[2] 沈岳良[2] 陈莹莹[2] 

机构地区：[1]浙江大学基础医学国家理科人才培养基地,浙江杭州310058 [2]浙江大学医学院生理教研室,浙江杭州310058

出　　处：《中国病理生理杂志》2009年第2期280-284,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30400094)

摘　　要：目的:研究葛根素对抗急性高糖刺激引起的血管舒张功能的下降,并分析血红素加氧酶(HO-1)在其中的作用。方法:采用血管环灌流装置,观察大鼠胸主动脉环的舒张效应。结果:①与空白对照组(含11mmol/L葡萄糖)相比,经44mmol/L葡萄糖(高糖)孵育血管2h后,主动脉环对ACh引起的内皮依赖性血管舒张反应下降。②葛根素(10-10-10-8mol/L)与高糖联合孵育,可剂量依赖性地改善高糖诱导的血管ACh舒张反应的下降。③葛根素孵育血管后可引起血管HO-1活性增高;用ZnPPIX抑制HO-1的活性后,葛根素抗高糖损伤的作用被取消。结论:葛根素可以对抗高糖引起的血管舒张功能的下降,其机制可能是通过诱导HO-1而实现的。AIM： To investigate the effect of puerarin on acute high glucose - induced attenuation of ACh relaxation in isolated rat aortic rings, and to elucidate its underlying mechanism. METHODS： The thoracic aortic rings with endothelium of male Sprague - Dawley rats were mounted on a bath system. Isometric relaxation of aortic rings was measured. RESULTS： ① After incubation with high concentration of glucose （44 mmol/L）, the vascular relaxation responses to ACh decreased. ② After coincubation with puerarin （ 10^-10 - 10^-8 mol/L） and high glucose, the high glucose - induced attenuation of ACh relaxation responses of artery was partly inhibited in a dose - dependent manner. ③ After incubation with puerarin for 2 h, the HO -1 activity of thoracic aorta increased. ZnPPIX （ an inhibitor of heme oxygenase - 1 ） abrogated the protective effect of puerarin. CONCLUSION： Puerarin prevents the acute high glucose -induced attenuation of endothelium - dependent relaxation in aortic rings. The mechanism might be involved in the activation of heme oxygenase - 1.

关 键 词：高糖血症 葛根素 血红素氧化酶 主动脉环 血管舒张 

分 类 号：Q463[生物学—生理学]