二酰亚胺类G-四链体配体抑制白血病细胞增殖及其分子机制  

Inhibition Effect of Diimide G-quadruplex Ligand on Proliferation of Leukemia Cells and Its Molecular Mechanisms

作　　者：褚彬[1] 袁谷[2] 周江[2] 欧媛[1] 朱平[1] 

机构地区：[1]北京大学第一医院血液研究室,北京100034 [2]北京大学化学与分子工程学院化学生物学系,北京100871

出　　处：《中国实验血液学杂志》2009年第1期43-48,共6页Journal of Experimental Hematology

基　　金：国家科技部国际科技合作重大项目(批准号:2006DFB31430);国家自然科学基金(批准号:30470939)

摘　　要：为了解二酰亚胺类G-四链体配体对白血病细胞的增殖抑制作用及其分子机制,用不同浓度(0.1-10μmol/L)的二酰亚胺类G-四链体小分子配体处理体外培养的白血病细胞系K562,用台盼蓝染料排斥法了解小分子对K562细胞生长曲线的影响,脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡,端粒重复序列扩增法(TRAP)检测端粒酶活性变化,基因微阵列技术(Microarray)分析基因表达谱的变化,逆转录-聚合酶链反应(RT-PCR)验证基因表达谱的部分结果。结果表明,二酰亚胺类小分子能够抑制K562细胞的增殖并诱导其凋亡。二酰亚胺类小分子处理后白血病细胞中端粒酶活性降低,细胞凋亡相关基因、信号通路成员基因、原癌基因等重要生物学功能相关的基因转录水平发生显著变化。结论:二酰亚胺类G-四链体配体是一种能够抑制白血病细胞增殖、诱导其凋亡的小分子,这种抗肿瘤效应可能是通过抑制端粒酶活性和调节某些重要基因的表达而实现的。This study was aimed to investigate the growth inhibition effect of diimide G-quadruplex ligand on leukemia cells and to explore its molecular mechanisms. K562 leukemia cell lines were treated with various concentrations of the diimide G-quadruuples ligand small molecule（0.1 -10 μmol/L）. Trypan blue exclusion assay was used to evaluate the proliferation inhibition. Cell apoptosis was observed using terminal deoxynucleotide transferase-mediated dUTP nick end labeling （TUNEL）. Telomerase activity was analyzed by telomere repeat amplification protocol. Gene expression was detected by microarray and confirmed by RT-PCR assay. The results showed that diimide small molecule inhibited the proliferation of K562 cells and induced apoptosis of these cells. After treating with diimide G-quadruplex ligand, telomerase activity of K562 cells was reduced and the transcriptional levels of some important genes were changed significantly. These genes were involved in cell apoptosis, cell signaling pathway and other key functions. In conclusion, the diimide G-quadruplex ligand is a small molecule that inhibits the proliferation and induces apoptosis in leukemia cells, and these functions may be related to telomerase inhibition and regulation of some important gene transcription.

关 键 词：二酰亚胺类G-四链体配体 白血病 细胞增殖 基因微阵列 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]