藤黄酸诱导Raji细胞凋亡的机理研究  

Mechanism of Gambogic Acid-induced Apoptosis in Raji Cells

作　　者：王勇[1,2] 陈燕 陈子[1] 柯文娟[1] 吴秋玲[1] 何静[1] 

机构地区：[1]华中科技大学同济医学院协和医院血液病研究所,湖北武汉430022 [2]九江学院附属医院血液肿瘤科,江西九江332000

出　　处：《中国实验血液学杂志》2009年第1期88-91,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目,编号30472267

摘　　要：本研究观察藤黄酸对Raji细胞的诱导凋亡作用,探讨死亡诱导清理子-1(death inducer-obliterator 1,DIO-1)在该过程中的作用。采用Annexin V-FITC/碘化丙锭(PI)染色流式细胞术检测细胞凋亡率,免疫印迹法检测Bcl-xL,DIO-1和pro-Caspase 3及其活化片段P17、P20的表达。免疫荧光化学和Hoechst 33258双标染色法检测DIO-1核转位。结果表明,藤黄酸呈剂量依赖性诱导Raji细胞凋亡,下调Bcl-xL表达并上调DIO-1与pro-Caspase 3片段表达,诱导Caspase活化片段的产生及DIO-1的核转位。结论:藤黄酸能诱导Raji细胞凋亡,其机制与DIO-1表达上调及核转位有关,可能通过Caspase 3活化而实现,DIO-1表达上调可能与Bcl-xL表达下调有关。This study was purposed to explore the apoptotic effect of gambogic acid on Raji cells and the role of death inducer-obliterator 1 （DIO-1） in this process. Annexin V-fluorescein-isothiocyanate/propidiurn iodide was used to detect apoptosis of Raji cells. Western blot was used to determine the expressions of DIO-1, Bcl-xL, pro-caspase 3 and 2 activated subunits： P17 and P20. The subcellular localization of DIO-1 in untreated and treated Raji cells was checked by immunofluorescence and Hoechst 33258 double staining. The results showed that the Gambogic acid dose-dependently induced the apoptosis of Raji cells, downregulated the expression of Bcl-xL, upregulated the expressions of DIO-1 and pro-caspase 3, induced the cleavage of pro-caspase 3 and DIO nuclear translocation. It is concluded that gambogic acid induces the apoptosis of Raji cells through DIO-1 upregulation, nuclear translocation, Bcl-xL downregulation and caspase 3 activation.

关 键 词：藤黄酸 细胞凋亡 死亡诱导清理子 BCL-XL RAJI细胞 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]