脑活素对液压冲击伤后原代神经细胞内游离钙的影响  

Effects of cerebrolysin on intracellular calcium in primary cultured neurons after fluid percussion injury

作　　者：黄慧玲[1] 武俏丽[1] 王辰[1] 王琼[1] 张文治[1] 苏心[1] 

机构地区：[1]天津市神经外科研究所,天津300060

出　　处：《中国药理学通报》2009年第2期205-209,共5页Chinese Pharmacological Bulletin

基　　金：天津市应用基础重点资助项目(No013803111)

摘　　要：目的探讨脑活素对液压冲击伤后神经细胞内游离钙的作用。方法原代培养的神经细胞分(1)正常组,(2)创伤组:液压冲击致神经细胞创伤后24h和48h组,(3)脑活素治疗组:分别于神经细胞创伤后0、1h给予1.25ml.L-1脑活素治疗(0h给药和1h给药组)。用激光扫描共聚焦显微镜和Ca2+荧光探针Fluo-3/AM观察细胞创伤后24h和48h细胞内游离钙[Ca2+]i的变化。结果神经元细胞创伤后,伤后24h神经元胞质内钙离子浓度达高峰,48h仍维持在较高水平;脑活素0或1h治疗后,在24h均能明显减轻创伤神经元细胞内的钙超载,48h后只有1h给予脑活素的细胞钙离子强度降低。结论脑活素对创伤的神经细胞有保护作用,且具有治疗时间窗。Aim cellular calcium in To investigate the changes Of intraprimary cultured neurons after fluid percussion injury under the effects of cerebrolysin. Methods The neurons of rats were divided into ： norreal group, FPI group and cerebrolysin group （ 0 h and 1 h treatment after fluid percussion injury （FPI）） . The intracellular calcium （ [ Ca^2 ＋] i ） at rats neurons in 24 h and 48 h postinjury were measured by using the laser scanning confocal microscope under calcium fluorescent indicator Fluo-3/AM. Results The [ Ca2 ＋ ] i at rats neurons were markedly increased after 24 h postinjury compared with normal neurons and maintained the higher level after 48 h. Cerebrolysin, whenever added at Oh or 1h after FBI, could significantly decrease the rise of [ Ca^2 ＋ ] i on 24 h postinjury, which only happened in 48 h postinjury by 1 h treatment after FPI. Conclusion Cerebrolysin has the protective effects on primary cultured rat cortical neurons of rats and has the time - window treatment.

关 键 词：脑活素 钙离子 神经细胞 激光共聚焦显微镜 

分 类 号：R-332[医药卫生] R322.81