酒精性肝病患者抗丙醛-乙醛加合物循环抗体滴度的测定  

The measurement of circulating antibodies against malondialdehyde-acetaldehyde adducts titers in patients with alcohol-induced liver disease

作　　者：杨永红[1] 骆岚[1] 夏培君[1] 张梗林[1] 

机构地区：[1]山东中医药大学第二附属医院消化内科,山东济南250001

出　　处：《胃肠病学和肝病学杂志》2009年第2期117-120,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：济南铁路局科委资助项目(02Y41-6)

摘　　要：目的探讨丙醛-乙醛加合物(MAA)是否参与了酒精性肝病(ALD)患者的免疫反应。方法采用酶联免疫吸附试验(ELISA)检测40例酒精性肝病患者、40例非酒精性肝病患者、30例无肝损害的重度嗜酒者、40例健康对照的血清中MAA加合物的循环抗体,并评价MAA加合物的免疫反应。结果酒精性肝病患者抗MAA IgG滴度比健康对照组明显增加(平均OD值分别为0.42±0.23、0.10±0.04,P<0.001),非酒精性肝病及重度嗜酒者中抗HSA-MAA IgG抗体(平均OD值分别为0.14±0.08、0.13±0.08)与对照组相比没有显著性差异。在酒精性肝病患者中抗MAA抗体为阳性,而非酒精性肝病患者、重度嗜酒者和健康对照多数为阴性。结论MAA具有免疫原性,可引起肝脏自身免疫反应,导致肝损伤,是引起酒精性肝病的重要因素。Objective To examine whether MAA adducts might participate in the immune response associated with human alcohol-induced liver disease. Methods A MAA adduct detection assay has been developed and used to evaluate MAA antibody response, circulating antibodies against MAA adducts were evaluated in 40 patients with alcohol-induced liver disease （ ALD）, in 40 patients with non alcohol-induced liver disease （ NALD）, in 30 heavy drinkers （HD） without liver damage and in 40 healthy which controls by enzyme-linked immunosorbent assays （ELISA）. Results The test results of this initial study showed that only the patients with ALD were positive for antibody against MAA. The patients with NALD, the heavy drinkers and healthy controls were negative. Immunoglobulin G （IgG） reacting with MAA-modified proteins were significantly increased in the patients with alcohol-induced cirrhosis or hepatitis. Conclusion These results show the formation of MAA antigens during alcohol-induced liver disease and suggest their possible contribution to the development of immunologic reactions associated with alcohol-related liver damage.

关 键 词：酒精性肝病 丙醛-乙醛加合物 酶联免疫吸附试验 

分 类 号：R575[医药卫生—消化系统]