sIL-2R、mIL-2R及TNF-α在多发性硬化症免疫学发病机制中的作用  

Study on the Role of sIL-2R,mIL-2R and TNF-αin the Pathogeuesis of Multiple Sclerosis

作　　者：王丽华[1] 王维治[2] 张凤蕴[3] 宋振海[1] 

机构地区：[1]解放军211医院 [2]哈尔滨医科大学附属第二医院神经内科,哈尔滨150086 [3]哈尔滨医科大学免疫学教研室

出　　处：《中国神经免疫学和神经病学杂志》1998年第1期6-11,共6页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的探讨可溶性白细胞介素-2受体（sIL－2R）、膜白细胞介素-2受体（mIL－2R）和肿瘤坏死因于-α（TNF－α）与多发性硬化（MS）免疫学发病机制、病程及病情的关系。方法采用ELkA法检测了临床确诊的48例MS患者血清、28例CSF中sIL－ZR水平，用免疫荧光法检测28例MS患者血中mIL－2R的表达，用生物活性测定法检测28例MS患者PBMCs体外诱生TNF－α水平。结果MS患者组激素治疗前血清及CSF中sIL－1R和血中TNF－α水平显著高于对照组（NC组）（P＜0．01），其中急性复发组MS显著高于缓解组（P＜0．01）；治疗后血清SIL－2R及TNF－α水平较治疗前显著下降（P＜0．01），且二者水平仍显著高于NC组（P＜0.01）。而缓解组TNF－α水平低于NC组（P＜0．05）。MS组血中mIL－2R表达，急性复发期MS患者显著高于缓解期（P＜0．01）及NC组（P＜0．01），缓解期MS患者则又显著低于NC组（P＜0．01）。MS患者血中sIL－2R及TNF－α水平与病情显著相关而与病程无关，sIL－2R与TNF－α显著相关。结论sIL－2R，mIL－2R、TNF－α在MS免疫发病中可能起重要作用，为探讨MS免疫发病机制进一步提供了理论依据。Objective To investigate the levels of soluble interleukin-2 receptor (sIL-2R), membrane interleukin-2 receptor (mIL-2R) and tumor necrosis factor-a(TNF-α) and their effectsin the pathogenesis of multiple sclerosis (MS). Methods We examined sIL-2R level ofserum in 48 MS patients and 50 subjects with normal controls, and sIL-2R level of CSF in 28MS patients and in 30 control subjects, respectively, by ELISA； the activity of tumor necrosis factor-α (TNF-α)was assayed in 28 MS patients and 30 controls by bioassay. The expression of mIL-2R in serum in 28 MS patients and 30 controls was detected by immunofluorescence technique. Fourty-eight MS patients were diagnosed according to the standard ofclinically definite MS by Poser. Results (1) The sIL-2R levels of serum and CSF in MSpatients' group were significantly higher than those of control group (P<0. 01) Ithe sIL-2Rlevel of serum in relapsing patients was significantly higher than that in remitting patients(P<0. 01) i the sIL-2R level of serum in MS patients after treatment with steroid was lowerthan before treatment (P<0. 01) and was still higher than normal controls (P<0. 01). (2)The expression of mIL-2R in PBMCs in relapsing MS patients was significantly higher thanthat in remitting patients or controls (P<0. 01). (3) The level of TNF-α was significantlyelevated in relapsing MS patients than that in remitting patients (P<0. 01); the level ofTNF-α in MS patients after treatment with steroid lower than before treatment (P<0. 01),but TNF-α level of remitting MS patients was significantly lower than normal controls(P<0. 05 ). (4) TNF-α level in serum was closely related to sIL-2R level in patients with MS.(5) There was no correlation between serum levels of sIL-2R or TNF-α and duration of thedisease. Conclusion Our experiments suggested that the changes of sIL-2R, mIL-2R andTNF-α levels in MS patients have reflected the disorders of immunological regulation; higherlevels of both sIL-2R and TNF-α have indicated the immune activation leading to persistenttr

关 键 词：白细胞介素-2 受体 肿瘤坏死因子 多发性硬化症 

分 类 号：R744.51[医药卫生—神经病学与精神病学]