荧光定量PCR检测非小细胞肺癌外周血Survivin-mRNA的临床意义  

Clinical significance of detecting apoptotic inhibitor gene Survivin in peripheral blood of non small cell lung cancer by real-time PCR

作　　者：白桂颖[1] 

机构地区：[1]天津市第三中心医院血液科,天津300170

出　　处：《武警医学院学报》2009年第1期37-39,42,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】探讨凋亡抑制基因Survivin在非小细胞肺癌外周血中表达的临床意义。【方法】对37例非小细胞肺癌患者分别采用荧光定量PCR方法检测其外周血Survivin-mRNA,采用免疫组化方法检测其肿瘤组织的Sur-vivin蛋白。【结果】肿瘤组织中Survivin蛋白阳性表达率为59.46%,外周血Survivin mRNA阳性率为64.86%。治疗前外周血SurvivinmRNA、治疗前、后SurvivinmRNA含量差值的含量在不同性别、年龄、治疗组及病理类型间差异无统计学意义(P>0.05),而在有无淋巴结、远处转移及最大肿瘤直径组差异有统计学意义(P<0.05)。治疗前、后SurvivinmRNA含量差值在不同疗效差异有统计学意义。【结论】非小细胞肺癌患者的外周血SurvivinmRNA检测可以替代瘤组织Survivin蛋白的检测。[ Objective] To investigate the relation of Survivin mRNA in peripheral blood of non small cell lung cancer （NSCLC） patient. [Methods] Both Survivin mRNA in peripheral blood by real-time PCR and protein in tumor tissue by immunohistochemical method were jointly detected in 37 patents with NSCLC. [Results] Positive rate of Survivin protein was 59.46%, and that of Survivin mRNA was 64.86% .No significant difference was found in positive rate of the level of Survivin mRNA before treatment and ΔSurvivin mRNA （difference of Survivin mRNA amount between pre-and post-therapy） in different sex, age and patho logical type groups （ P 〉 0.05 ）.However, significant difference was found between level of Survivin mRNA before treatment or Survivin InRNA in different diameter of tumor , non and existed lymphatic or diatant metastases （P 〈 0.05） .3.Positive correlation was found between the level of Survivin mRNA in peripheral blood of NSCLC before operation and Survivin protein in tumor tissue. [ Conclusions] It suggests that the detectoin of SurvivinmRNA in peripheral blood may replace the analysis of Survivin protein in tissue.

关 键 词：非小细胞肺癌 生存素 RT-PCR 疗效 

分 类 号：R734.2[医药卫生—肿瘤]