康氏木霉REMI突变株的差异表达蛋白分析(简报)  

ANALYSIS OF DIFFERENTIAL PROTEIN EXPRESSION OF REMI MUTANT OF Trichoderma koningii

作　　者：胡仕凤[1,2] 朱木兰[3] 刘力行[2] 唐俊[2] 高必达[1] 陈捷[2] 

机构地区：[1]湖南农业大学,长沙410128 [2]上海交通大学农业与生物学院,上海200240 [3]中国科学院上海植物生理生态研究所,上海200032

出　　处：《分子细胞生物学报》2009年第1期77-81,共5页Journal of Molecular Cell Biology

基　　金：国家科技支撑课题(2006BAD08A06);农业部948项目(编号:2006-G54(A));上海市科委中法合作项目(063407060)

摘　　要：纤维素占植物干重的35％-50％，是地球上分布最广、含量最丰富的碳水化合物，它的利用与转化对于解决目前世界能源危机、粮食短缺、环境污染等问题具有十分重要的意义。因此，产纤维素酶微生物的基因工程改造技术受到很大关注，成为当前研究热点之一。On purpose of expounding the phenotype change of Trichoderma mutant which produces cellulase, protein profiles of T. koningii CICC and its mutant TK-2R-1 were detected by 2- DE. Eight unique spots were found and four of them were identified and their functions by MSTOF-TOF were connected with growth and metabolization of Trichoderma spp. The research provides a new theoretical basis and methods for the construction of Trichoderma mutant which is used to produce efficient cellulase.

关 键 词：康氏木霉 纤维素酶 REMI技术 双向电泳 蛋白质 

分 类 号：Q93[生物学—微生物学]