2株鲤鱼源乳酸菌体外抗逆性研究  

Study on Resistance of Two Carp Lactobacillus in Vitro

作　　者：单晓枫[1] 孙烨林 张洪波[1,3] 张喜宏[1] 钱爱东[1,3] 

机构地区：[1]吉林农业大学预防兽医学学科,吉林长春130118 [2]长春市动检站,吉林长春130118 [3]吉林农业大学动物生产及产品质量安全教育部重点实验室,吉林长春130118

出　　处：《水生态学杂志》2008年第6期124-127,共4页Journal of Hydroecology

基　　金：国家自然科学基金资助项目(30471286)

摘　　要：对2株鲤鱼源乳酸菌作为益生菌候选菌株进行体外抗逆性试验。通过耐受胆盐、耐受pH值、耐受蛋白酶和耐高温等试验,对这2株乳酸菌进行研究。其中在胆盐耐受性试验中,YL-1和YL-7在胆盐浓度0.4%、培养4h时仍有30%以上的存活率;在pH值耐受性试验时,2株乳酸菌均可在一定的酸性和碱性条件下存活;胰蛋白酶对2株菌的存活率没有影响;在70℃的高温中,2株乳酸菌均有一定的耐受性。Resistance of two carp -source lactobacillus was performed , which were the candidate probiotic strain. They were researched through the test of tolerance of cholate, tolerance of pH, tolerance of protease , tolerance of high temperature and so on. Among all the tests, over 30% of YL - 1 and YL -7 could survive after culture 4 hours and in 0.4% concentration in tolerance of cholate test. And in tolerance of pH test, 2 strains of Lactic Acid Bacteria could survive both in acid environment and alkaline environment. Trypsin did not affect the survival of them, Moreover, they showed certain tolerance in the megatemperature of 70℃.

关 键 词：鲤鱼 乳酸菌 抗逆性 

分 类 号：S917[农业科学—水产科学]