细胞色素c在硅基有序介孔材料SBA-15上直接电化学行为  

Direct Electrochemistry of Cyt c on Ordered Mesoporous Silica SBA-15 Modified Glassy Carbon Electrode

作　　者：徐继明[1] 张瑞娟[2] 刘正会[1] 尹起范[1,2] 

机构地区：[1]江苏省低维材料化学重点建设实验室淮阴师范学院化学系,江苏淮安223300 [2]延边大学理学院化学系,吉林延吉133002

出　　处：《淮阴师范学院学报（自然科学版）》2008年第4期291-294,共4页Journal of Huaiyin Teachers College;Natural Science Edition

基　　金：江苏省教育厅自然科学基金资助项目(06KJB150011)

摘　　要：用水热法合成了硅基有序介孔分子材料SBA-15,研究了细胞色c(Cyt c)在SBA-15修饰玻碳(GC)电极表面的直接电化学行为.结果表明,在pH 7.0磷酸缓冲溶液中(PBS),细胞色素c在SBA-15修饰电极表面表现出一对准可逆的氧化还原峰,电位差ΔE为87 mV,式电位E0′为0.275 V,峰电流的大小与循环伏安扫描速度成正比,说明细胞色素c在电极表面过程是受表面控制.固定在电极上的细胞色素c能促进过氧化氢的催化还原,显示出较高的亲和力,为过氧化氢传感器的研制提供理论依据.Mesoporous SBA-15 was synthesized by using a hydrothermal method. Cytochrome c was immobilized on SBA-15 modified glassy carbon electrode. The interaction between Cytochrome c and SBA-15 was examined by using UV-Vis spectroscopy and electrochemical methods. The direct electron transfer of the immobilized Cytochrome c exhibited a pair of redox peaks with formal potential （ E^0 ） of about 0.275 V （versus SCE） in 0. lmol/L pH 7.0 PBS. The electrode reaction showed a surface-controlled process with the apparent heterogeneous electron transfer rate constant （ks） of 2.25 s^-1. Cytochrome c immobilized on the SBA-15 modified electrode exhibits a remarkable electrocatalytic activity for the reduction of hydrogen peroxide （H2O2 ）. Based on these, a third generation biosensor can be constructed for the determination of H2O2.

关 键 词：介孔分子材料 细胞色素C 生物传感器 直接电子转移 过氧化氢 

分 类 号：O657.1[理学—分析化学]